关键词: 颅骨缺损, 辛伐他汀, 同轴静电纺丝, 引导骨再生技术（GBR）

Abstract: Objective　 To observe the effect of PCL-GT/PCL-simvastatin membrane on repairing defect of rabbit skull. Methods: PCL-GT/PCL-simvastatin membrane was prepared, and the surface morphology was observed by scanning electron microscopy. The sustained release of simvastatin was detected. Eighteen male New Zealand white rabbits were randomly divided into 3 groups with 6 rabbits in each group to prepare skull defect model. The defect area of blank group was not implanted with membrane; the defect area of negative control group was implanted with PCL-GT/PCL membrane; the defect area of experimental group was implanted with PCL-GT/PCL-simvastatin membrane. Animals in each group were sacrificed at 4 and 12 weeks after operation, respectively. MicroCT and HE staining were performed. Results: Results of scanning electron microscopy showed that the obtained PCL-GT/PCL-simvastatin membrane was porous with different pore sizes in different tissue surfaces. Simvastatin could be released stably and slowly for up to 28 days. MicroCT and HE staining results showed that the new bone formation and bone defect healing in the experimental group were significantly better than those in the negative control group and the blank group (P< 0.05). Conclusion: The experiment shows that PCL-GT/PCL-simvastatin membrane can significantly promote osteogenesis, accelerate the repair of bone defects, and improve repair quality.

Key words: Skull defect, Simvastatin, Coaxial electrospinning, Guided bone regeneration technology (GBR)