可注射型富血小板纤维蛋白对人根尖牙乳头干细胞生物学行为的影响

›› 2021, Vol. 41 ›› Issue (7): 594-598.

可注射型富血小板纤维蛋白对人根尖牙乳头干细胞生物学行为的影响

潘菁,曹子蔚,管贇,蒋备战

同济大学附属口腔医院/口腔医学院；上海牙组织修复与再生工程技术研究中心

收稿日期:2021-01-04 修回日期:2021-02-28 出版日期:2021-07-28 发布日期:2021-07-28
通讯作者: 蒋备战 E-mail:jiangbeizhan@tongji.edu.cn
基金资助:
上海市科学技术委员会科普专项;上海市科学技术委员会医学引导项目;中央高校基本科研业务费专项资金

Effect of injectable platelet rich fibrin on human stem cells from apical papilla

Received:2021-01-04 Revised:2021-02-28 Online:2021-07-28 Published:2021-07-28
Contact: Beizhan /Jiang E-mail:jiangbeizhan@tongji.edu.cn

摘要/Abstract

摘要： 目的探讨可注射型富血小板纤维蛋白（injectable platelet rich fibrin，iPRF）对人根尖牙乳头干细胞（human stem cells from apical papilla，hSCAPs）生物学行为的影响。方法酶联免疫吸附实验（enzyme linked immunosorbent assay，ELISA）测定可注射型富血小板纤维蛋白提取液（injectable platelet rich fibrin extract，iPRFe）生长因子的含量；CCK8、Transwell实验、茜素红染色分别检测iPRFe对hSCAPs增殖、迁移、矿化诱导的影响；荧光实时定量PCR检测与成骨/成牙向分化相关基因的mRNA表达水平。结果 iPRFe中可检测到血小板衍生生长因子BB（platelet derived growth factorBB，PDGFBB)、胰岛素样生长因子1（insulinlike growth factor 1，IGF1）和转化生长因子β1（transforming growth factor β1，TGFβ1）；iPRFe能促进hSCAPs的增殖，且在1/4×浓度时hSCAPs增殖效果最佳；1/4×浓度的iPRFe对hSCAPs的迁移和矿化也有明显的促进效果；iPRFe还可明显促进碱性磷酸酶（alkaline phosphatase，ALP）、牙本质基质蛋白1（dentin matrix protein 1，DMP1）、牙本质涎磷蛋白（dentin sialophosphoprotein，DSPP）和骨钙素（osteocalcin，OCN） mRNA的表达（P<0.05）。结论 iPRF能够促进hSCAPs的增殖、迁移和矿化，有望为牙髓再生提供可能。

关键词: 可注射型富血小板纤维蛋白, 根尖牙乳头干细胞, 生物学行为, 牙髓再生

Abstract: Objective To explore the effects of injectable platelet rich fibrin on the biological behavior of human stem cells from apical papilla (hSCAPs)in vitro. Methods The content of growth factors in injectable platelet rich fibrin extract (iPRFe) was quantified by enzyme linked immunosorbent assay(ELISA). The effects of iPRFe on the proliferation of hSCAPs were detected by CCK8 and the migration of hSCAPs was evaluated via Transwell assay. The mineralized nodules were observed by alizarin red staining and the realtime PCR was used to explore the expression levels of odonto/osteogenicrelated mRNA. Results Platelet derived growth factorBB (PDGFBB), insulinlike growth factor 1 (IGF1) and transforming growth factor β1 (TGFβ1)were detected in iPRFe. iPRFe promoted the proliferation of hSCAPs, and the optimum concentration for cell proliferation was 1/4×. The migration and mineralization of hSCAPs were also promoted by 1/4× concentration of iPRFe. iPRFe significantly promoted mRNA expression levels of alkaline phosphatase (ALP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP) and osteocalcin (OCN)(P<005). Conclusion iPRF can promote the proliferation, migration and mineralization of hSCAPs, which is expected to provide possibility for pulp regeneration.

Key words: injectable platelet rich fibrin, stem cells from apical papilla, biological behaviour, pulp regeneration

中图分类号:

R781.05

潘菁曹子蔚管贇蒋备战. 可注射型富血小板纤维蛋白对人根尖牙乳头干细胞生物学行为的影响[J]. 口腔医学, 2021, 41(7): 594-598.

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