哺乳动物雷帕霉素靶蛋白在牙周炎大鼠相关肾损伤中的作用

doi:10.13591/j.cnki.kqyx.2022.05.007

摘要/Abstract

摘要： 目的本研究旨在初步探讨哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)在牙周炎大鼠相关肾损伤中的作用。方法 20只雄性Wistar大鼠被随机分为对照组和牙周炎组,每组各10只。牙周炎组采用0.2 mm正畸丝结扎法构建牙周炎动物模型,对照组不作结扎处理。8周后,分别记录两组大鼠牙龈出血指数、牙周袋探诊深度及松动度,并通过Micro-CT分析牙槽骨吸收情况;采用HE及PAS染色进行组织学分析。qRT-PCR和Western Blot分别检测大鼠肾组织内的mTOR、phosphor-mTOR(p-mTOR)和BECN1(Beclin-1)的mRNA及蛋白表达情况。结果与对照组对比,牙周炎组大鼠的牙龈组织出现明显肿胀、颜色及质地的改变,牙龈出血指数、牙周袋探诊深度及松动度均明显增加,牙槽骨吸收明显;肾小球鲍曼氏囊腔明显扩张,基底膜增厚,肾小管间质充血明显,刷状缘破坏;肾脏功能未见明显差异;牙周炎组的mTOR的mRNA及蛋白表达(p-mTOR/mTOR)均明显高于对照组,Beclin-1的mRNA及蛋白表达均明显低于对照组。结论 mTOR可能通过抑制自噬过程在大鼠牙周炎相关肾损伤中发挥作用。

关键词: 牙周炎, 肾损伤, 哺乳动物雷帕霉素靶蛋白

Abstract: Objective To investigate the role of mammalian target of rapamycin (mTOR) in renal injury in rats with periodontitis. Methods Twenty male Wistar rats were randomly divided into control group and periodontitis group, with 10 rats in each group. The animal model of periodontitis group was established by 0.2 mm orthodontic wire ligation, and ligation was not conducted in the control group. After 8 weeks, gingival bleeding index, probing depth of periodontal pocket were, and loosening degree recorded and alveolar bone resorption was analyzed by Micro-CT. HE and PAS staining were used for histological analysis. qRT-PCR and Western Blot were used to detect the mRNA and protein expression of mTOR, phosphor-mTOR (p-mTOR) and BECN1 (Beclin-1) in kidney tissues of rats. Results Compared with the control group, the gingival tissue in periodontitis group showed obvious swelling, color, and texture changes, and the gingival bleeding index, the probing depth of periodontal pocket and the degree of loosening were significantly increased and alveolar bone resorption was obvious. The glomerular Bowman's capsular space was obviously dilated; basement membrane hyperplasia, renal tubule interstitial hyperemia was obvious, and brush margin was destroyed; there was no significant difference in renal function.The mRNA and protein expressions (p-mTOR/mTOR) of mTOR in periodontitis group were significantly higher than those in control group, and the mRNA and protein expressions of Beclin-1 were significantly lower than those in control group. Conclusion mTOR may play a role in periodontitis-related renal injury in rats by inhibiting autophagy.

Key words: periodontitis, renal injury, mammalian target of rapamycin

中图分类号:

R781.4

丁旭, 李鑫, 刘歆婵, 侯玉帛, 武洲, 于维先. 哺乳动物雷帕霉素靶蛋白在牙周炎大鼠相关肾损伤中的作用[J]. 口腔医学, 2022, 42(5): 420-424.

DING Xu, LI Xin, LIU Xinchan, HOU Yubo, WU Zhou, YU Weixian. Role of mammalian target of rapamycin in renal injury associated with periodontitis in rats[J]. Stomatology, 2022, 42(5): 420-424.

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