2型糖尿病对大鼠牙周组织及血清TNF-α、IL-6和PAI-1水平影响的研究

›› 2017, Vol. 37 ›› Issue (10): 889-893.

2型糖尿病对大鼠牙周组织及血清TNF-α、IL-6和PAI-1水平影响的研究

王利宏¹,张文彪¹,习利军¹,包爱琴¹,黄世光²

1. 深圳市福田区中医院
2. 暨南大学

收稿日期:2016-12-26 修回日期:2017-02-20 出版日期:2017-10-28 发布日期:2017-10-13
通讯作者: 黄世光 E-mail:thshg@126.com
基金资助:
广东省深圳市福田区科技创新项目

The influence of type 2 diabetic rats with periodontitis on histological changes in periodontal tissue and serum levels of TNF-α, IL-6 and PAI-1

Received:2016-12-26 Revised:2017-02-20 Online:2017-10-28 Published:2017-10-13
Contact: Shiguang Huang E-mail:thshg@126.com

摘要/Abstract

摘要： 目的　观察2型糖尿病伴牙周炎大鼠牙周组织及血清TNF-α、IL-6及PAI-1水平的变化，探讨TNF-α、IL-6及PAI-1的表达对2型糖尿病牙周炎大鼠牙周组织改建过程中组织变化的影响。方法：清洁级8-9周龄Wistar大鼠60只，分为4组：○1伴牙周炎糖尿病组(n=15);○2不伴牙周炎糖尿病组(n=15);○3伴牙周炎非糖尿病组(n=15);○4不伴牙周炎非糖尿病组(即正常对照组，n=15)。采用腹腔内注射链脲佐菌素(streptozotocin, STZ)法建立大鼠糖尿病模型。给药后每周1次，连续4周测定大鼠尾静脉血糖水平，以判断大鼠糖尿病建模情况。用浸有牙龈卟啉单胞菌株的丝线结扎左侧上颌第二磨牙牙颈部，复制实验性牙周炎模型。实验性牙周炎建模20周后，采用腹腔注射水合氯醛法在全麻状态下处死大鼠。从腹主动脉采集血液，ELISA法检测血清TNF-α、IL-6及PAI-1水平。处死前测量术区的牙周附着情况。制作牙体牙周联合切片，观察牙周组织学改变。结果：糖尿病组大鼠表现为血糖和体重水平显著升高(P<0.05)；糖尿病组大鼠血清胰岛素水平和胰岛素抵抗指数得分均显著升高(P<0.05)；用浸有牙龈卟啉单胞菌株的丝线结扎后，牙周炎组表现为典型的牙周炎临床特征；与不伴牙周炎糖尿病组相比，伴牙周炎糖尿病组大鼠血清TNF-α、IL-6及PAI-1水平显著升高(P<0.05)。结论：糖尿病通过促进血清炎性因子的表达，降低牙周组织对局部致病因子的抵抗力，从而加重、加速牙周炎的进展。

关键词: 2型糖尿病, 牙周炎, 肿瘤坏死因子-α, 白细胞介素-6, 纤溶酶原活化物抑制因子-1

Abstract: Objective　To observe the histological changes in periodontal tissue and the serum levels of TNF-α, IL-6 and PAI-1 in type 2 diabetic rats with periodontitis, and to investigate the influence of expression of TNF-α, IL-6 and PAI-1 on the histological changes of periodontal tissue remodeling in periodontitis rats with type 2 diabetes mellitus. Methods: Sixty SD Wistar rats aged 8-9 weeks were divided into four groups according to different modeling methods: ○1diabetic rats with periodontitis group(n=15);○2diabetic rats without periodontitis group(n=15); ○3non-diabetic rats with periodontitis group(n=15); ○4non-diabetic rats without periodontitis group(n=15). Diabetic model was established by intraperitoneal injection of streptozotocin(STZ). Diabetes was determined by measuring blood glucose in rat tail vein once a week after giving the drug and for four successive weeks . The rat experimental periodontitis was induced by wrapping 3/0 silk ligature inoculated with Porphyromonasgingivalis around the left maxillary second molar of the rats and then the model was copied. The rats were sacrificed at 20th week after ligature by intraperitoneal injection of chloral hydrate . The blood was collected from aorta abdominalis. Then serum levels of TNF-α, IL-6 and PAI-1 were measured with ELISA method. The attachment loss(AL) was measured before sacrifice. The histological changes of periodontal tissues were observed under microscope with HE staining. Results: The diabetic rats showed a significant increase in blood glucose and weight(P<0.05).The serum insulin level and insulin resistance values of the diabetic rats significantly increased(P<0.05). The rat experimental periodontitis showed typical clinical characteristics of periodontitis after ligature inoculated with Porphyromonasgingivalis. The levels of serum TNF-α, IL-6 and PAI-1 were significantly higher in diabetic rats with periodontitis than those in diabetic rats without periodontitis group(P<0.05). Conclusion: Diabetes can decrease the periodontal tissue resistance to local pathogenic factors by promoting the expression of serum inflammatory factors, thereby further aggravating and accelerating the progress of periodontitis.

Key words: Type 2 diabetes mellitus, Periodontitis, Tumor necrosis factor-alpha, Interleukin-6, Plasminogen activator inhibitor-1

中图分类号:

R781.4

王利宏张文彪习利军包爱琴黄世光. 2型糖尿病对大鼠牙周组织及血清TNF-α、IL-6和PAI-1水平影响的研究[J]. , 2017, 37(10): 889-893.

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