关键词: 【关键词】TiO2纳米管, 种植体, 载银, 炎症细胞, TNF-α

Abstract: Objective To investigate the effect of silver loaded titanium dioxide (TiO2) nanotube implant and its effect on the inflammatory response of surrounding tissues. Methods The planting nails were divided into pure titanium polishing group (PT group), TiO2 nanotube group (NT group), low concentration of Ag loaded TiO2 nanotube group (NAg-L group) and high concentration of Ag loaded TiO2 nanotube group (NAg-H group), which were implanted into the bilateral mandible of adult dogs. The gingival crevicular fluids of every group were collected and their volume changes were analyzed. The Infiltration condition of inflammatory corpuscle in soft tissue was observed by HE staining, and the expression of TNF-? mRNA around soft tissue was detected by real time quantitative PCR. The morphological changes of adherent cells at different times were observed by electron microscope. Results When tested for 7d,the gingival crevicular fluid volume of NAg-L group was significantly lower than that of other groups(P＜0.05);when tested for 14d,the gingival crevicular fluid volume of NAg-H group was significantly lower than that of PT group(P＜0.05);when tested for 28d,there was no significant difference in gingival crevicular fluid volume in each group(P>0.05).The histological observation of each group at different times showed that PT group, compared with NT group, NAg-H group and NAg-L group, had more inflammatory cell infiltration. When tested for 7 d, the expression level of TNF-? mRNA in the NAg-L group was significantly lower than that of other groups (P <0.05), while the expression level of TNF-? mRNA in the NAg-H group was significantly higher than that of other groups (P <0.05). When tested for 14d,the expression level of TNF-? mRNA in the NAg-H group was significantly lower than that of other groups (P <0.05);the expression level of TNF-? mRNA in the NAg-L group was significantly lower than that of PT group and NT group(P <0.05).When tested for 28d,the expression level of TNF-? mRNA was not statistically significant among the groups (P >0.05).The surface adhesion cells in different groups at different times by electron microscope observation showed that when tested for 7d, there was no significant difference in the surface adherent cell of each group, and all presented star shaped protrusions; when tested for 14d, the cells of NT, NAg-H and NAg-L groups showed typical long spindle stretch of fibroblasts, while the cell of PT group had no typical morphology and small extension area; when tested for 28d,the surface cells of each group had good extension. Conclusion TiO2 nanotubes have better biocompatibility, which can make the soft tissue around the implant produce a smaller inflammatory response, and the low dose of Nano silver particles can significantly inhibit the inflammatory response.

Key words: [Keywords]TiO2 nanotube, implant, silver, inflammatory cells, TNF-α