有机溶剂去除牙胶对根管显微硬度的影响

摘要/Abstract

摘要： 目的比较丁克除，桉叶油，D-柠檬烯三种有机溶剂对根充后离体牙再通能力及根管牙本质显微硬度的影响，并观察3种有机溶剂对粪肠球菌的抑菌性能。方法选用40颗离体单根牙，去除冠方牙体组织，保证根管工作长度均为14mm，样本随机分为4组：A组（无溶剂组）,B组（丁克除组），C组（桉叶油组），D组（D-柠檬烯组）,每组10颗牙齿。体外根管充填后，比较各组不同溶剂处理下根管再通时间（T1和T2）、根尖推出物质量（g）、根管内残留物百分比（%），使用维氏显微硬度测试仪测量样本显微硬度值（HV，Mpa），纸片扩散法行药敏实验，测量抑菌圈直径并进行统计分析。结果三个有机溶剂组与对照组相比均能提高去除牙胶的效率（P <0.05），但三组内比较差异无统计学意义（P>0.05）。三个实验组牙本质显微硬度的变化与对照组比较无显着差异（P>0.05），但丁克除组降低根管牙本质显微硬度，D-柠檬烯组升高根管牙本质显微硬度，两组比较差异具有统计学意义（P <0.05）。D-柠檬烯和桉叶油对粪肠球菌均有抑制作用，D-柠檬烯抑菌作用强于桉叶油（P <0.05),丁克除未见明显的抑菌性。结论三种溶剂在去除牙胶效率方面，与对照组相比均能提高清除效率。D-柠檬烯较丁克除相比，可提高根管显微硬度。D-柠檬烯和桉叶油对粪肠球菌均有抑制作用，二者相比，D-柠檬烯的抑菌作用更强。

关键词: 显微硬度, 粪肠球菌, 丁克除, 桉叶油, D-柠檬烯

Abstract: Aim:The purpose of this study was to assess the efficacy of desocclusol, eucalyptol and d-limonene on removal of gutta-percha, their effect on microhardness of dentin, and the antibacterial properties against Enterococcus faecalis (E. faecalis) during root canal retreatment. Methods: Forty single-canal roots, obturated with gutta-percha, were prepared and randomly devided into 4 groups(n=10). Group that free of any solvent was control group.Three of experimental groups were exposed to desocclusol, eucalyptol and d-limonene respectively during endodontic retreatment. Efficacy of each solvent was assessed by comparation of duration time, amount of apical extrusion and residual debris in the root canals. Vickers microhardness of each sample was recorded. Antibacterial activity on E. faecalis was also observed. The data were subjected to repeated statistical analysis. Results: All three organic solvents were found to be effective in the removal of root canal filling material compared with control group(P<0.05). D-limonene was more effective in significantly increasing the microhardness of dentin compared with desocclusol, which decreased the microhardness of root canal dentin(P<0.05). In the meantime, d-limonene had a stronger antibacterial activity than eucalyptol, while desocclusol had no obvious bacterial inhibition. Conclusion: These three organic solvents are all effective in removing gutta-percha in endodontic retreatment. D-limonene can increase the microhardness of dentin and has a stronger inhibitory effects on E.faecalis than desocclusol and eucalyptol.

Key words: Microhardness, Enterococcus faecalis, Desocclusol, Eucalyptol, D-limonene.

中图分类号:

R781.3

李胜男潘爽沈帅王璐瑶张琳牛玉梅. 有机溶剂去除牙胶对根管显微硬度的影响[J]. , 2019, 39(7): 601-608.

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