含氟化物和氯己定牙膏的抗菌性能的体外研究

›› 2020, Vol. 40 ›› Issue (11): 1015-1020.

含氟化物和氯己定牙膏的抗菌性能的体外研究

陈玉莲¹,秦子玥²,陈武³

1. 南京医科大学附属口腔医院牙周科
2. 南京医科大学口腔疾病研究江苏省重点实验室
3. 江苏省口腔医院

收稿日期:2020-03-03 修回日期:2020-04-29 出版日期:2020-11-28 发布日期:2020-11-24
通讯作者: 陈武 E-mail:dentistchenwu@126.com

Study of antibacterial properties of a toothpaste containig both fluoride and chlorhexidine disgluconate in vitro

Received:2020-03-03 Revised:2020-04-29 Online:2020-11-28 Published:2020-11-24

摘要/Abstract

摘要： 目的研究含氟化物和氯己定双活性成分的牙膏与仅含氟化物的牙膏体外抗菌性能上的差异。方法测量不同牙膏对牙龈卟啉单胞菌（Porphyromonas gingivalis，P.gingivalis）、血链球菌（Streptococcus sanguis，S.sanguis）、变异链球菌（Streptococcus mutans，S.mutans）、大肠埃希氏菌（Escherichia coli，E.coli）、金黄色葡萄球菌（Staphylococcus aureus, S.aureus）作用产生的抑菌环大小。体外于直径6 mm的羟基磷灰石圆片（hydroxyapatites discs，HAs）表面培养含S.mutans、S.sanguis、P.gingivalis的三菌种生物膜，通过结晶紫染色及活死菌染色的方法来评价不同牙膏对抑制生物膜的作用。结果含氟化物和氯己定双成分的牙膏和仅含氟化物的牙膏对5种细菌均有一定的抑菌作用。对三菌种生物膜活性的作用，两种牙膏均较对照组有抑制作用。对生物膜的量及厚度的作用，含氟化物和氯己定的牙膏较PBS对照组明显减少。结论含氯己定和氟化物的牙膏较只含氟化物的牙膏体外抗菌效果好。

关键词: 牙膏, 氯己定, 氟化物, 生物膜

Abstract: Objective To compare the antibacterial properties of a toothpaste containing both fluoride and chlorhexidine with a toothpaste only containing fluoride. Methods The antibacterial ring sizes of different toothpastes against Porphyromonas gingivalis (P.gingivalis), Streptococcus sanguis (S.sanguis), Streptococcus mutans (S.mutans), Escherichia coli (E.coli), and Staphylococcus aureus (S. aureus) were measured. A threespecies biofilm model that consists of S.mutans, S.sanguis and P.gingivalis was established on the hydroxyapatites discs (HAs, d=6 mm) in vitro. The biofilms on the surface of HAs were detected by crystal violet staining and LIVE/DEAD bacteria staining to evaluate the antibiofilm properties of different toothpastes. Results Toothpastes with both fluoride and chlorhexidine and toothpaste with fluoride both had a certain antibacterial effect on five kinds of bacteria. The viability of the threespecies biofilms was significantly inhibited by two kinds of toothpastes. The biomass and thickness of the biofilms were significantly reduced by the toothpaste containing both fluoride and chlorhexidine. Conclusion Toothpastes containing both fluoride and chlorhexidine have greater effects on antibacterial properties than a conventional fluoridecontaining toothpaste.

Key words: Toothpaste, Chlorhexamine, Fluoride, Biofilm

中图分类号:

R988.2

陈玉莲秦子玥陈武. 含氟化物和氯己定牙膏的抗菌性能的体外研究[J]. 口腔医学, 2020, 40(11): 1015-1020.

参考文献 0

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