Taz及Runx2在大鼠下颌第一磨牙发育中的表达研究

Abstract

Abstract: Objective To investigate the spatial and temporal expression of transcriptional coactivator with PDZbinding motif(Taz) and Runtrelated transcription factor 2(Runx2) during the development of first molar in rat mandibular and analyze the function of Taz in tooth development and the interrelation between Taz and Runx2. Methods Samples were collected from Wistar rat at various stages. Immunohistochemical method was used to observe the expression pattern and localization of Taz and Runx2. Results At the bud stage, cap stage and early bell stage, the positive expression of Taz was detected in the oral epithelium and dental mesenchyme. With the development of tooth germ, the strongest staining of Taz appeared in early bell stage. Strongly positive expression of Taz was found in the inner enamel epithelium, outer enamel epithelium, stratum intermedium and dental follicle. The expression of Taz was positive in postnatal odontoblasts and predentin, and its expression level in ameloblasts decreased with the mineralization of enamel. Runx2 in different stages presented different expression and localization in embryonic samples. At the bud and cap stage, Runx2 was found in the oral epithelium and dental mesenchyme. At the early bell stage, positive staining was detected in the inner enamel epithelium, stratum intermedium, dental papilla and dental follicle, in addition to oral epithelium and dental mesenchyme. Runx2 expression in odontoblasts which was in secretory phase was positive and the staining gradually reduced with the differentiation of odontoblasts. Conclusion The expression of Taz during the development of first molars in rat mandibular is spatiotemporal. The result suggests that Taz may be an important regulatory factor in tooth development. The expression and localization of Taz was similar to Runx2, indicating that there is certain correlation between Taz and Runx2 in regulating tooth development of rat.

Key words: transcriptional coactivator with PDZ-binding motif, Runt-related transcription factor 2, tooth development, immunohistochemistry

CLC Number:

R392.114

Bing ZHANG. The expression of Taz and Runx2 in the first mandibular molar of developing rat[J]. , 2021, 41(7): 583-587.

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The expression of Taz and Runx2 in the first mandibular molar of developing rat

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