可注射型富血小板纤维蛋白对人根尖牙乳头干细胞生物学行为的影响

Abstract

Abstract: Objective To explore the effects of injectable platelet rich fibrin on the biological behavior of human stem cells from apical papilla (hSCAPs)in vitro. Methods The content of growth factors in injectable platelet rich fibrin extract (iPRFe) was quantified by enzyme linked immunosorbent assay(ELISA). The effects of iPRFe on the proliferation of hSCAPs were detected by CCK8 and the migration of hSCAPs was evaluated via Transwell assay. The mineralized nodules were observed by alizarin red staining and the realtime PCR was used to explore the expression levels of odonto/osteogenicrelated mRNA. Results Platelet derived growth factorBB (PDGFBB), insulinlike growth factor 1 (IGF1) and transforming growth factor β1 (TGFβ1)were detected in iPRFe. iPRFe promoted the proliferation of hSCAPs, and the optimum concentration for cell proliferation was 1/4×. The migration and mineralization of hSCAPs were also promoted by 1/4× concentration of iPRFe. iPRFe significantly promoted mRNA expression levels of alkaline phosphatase (ALP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP) and osteocalcin (OCN)(P<005). Conclusion iPRF can promote the proliferation, migration and mineralization of hSCAPs, which is expected to provide possibility for pulp regeneration.

Key words: injectable platelet rich fibrin, stem cells from apical papilla, biological behaviour, pulp regeneration

CLC Number:

R781.05

References 19

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Effect of injectable platelet rich fibrin on human stem cells from apical papilla

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