Stomatology ›› 2022, Vol. 42 ›› Issue (5): 399-403.doi: 10.13591/j.cnki.kqyx.2022.05.003

• Basic Research • Previous Articles     Next Articles

Effects of DLX5 on migration and chemotaxis of DT-MSCs

WU Taotao, ZHANG Yuheng, ZHENG Xiaoyu, LIU Chang, MA Huarui, GAO Runtao   

  1. Department of Stomatology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China
  • Revised:2022-01-12 Published:2022-05-24

Abstract: Objective To reveal the function of Distal-Less Homeobox 5 (DLX5) in migration and chemotaxis of dental tissue-derived mesenchymal stem cells (DT-MSCs). Methods Retrovirus was used to transfect stem cells from apical papilla (SCAPs). After constructing the plasmid of DLX5, we overexpressed and knocked down the expression of DLX5. Real-time RT-PCR and Western Blot were used to detect the expression of DLX5. Scratch migration assays and transwell chemotaxis assays were used to examine the migration and chemotaxis capacity of SCAPs in vitro. The effects of DLX5-overexpressing conditioned medium on the migration and chemotaxis capacities of PDLSCs were tested by Scratch migration assays and transwell chemotaxis assays in vitro. Results ①After transferring the plasmid of DLX5 overexpression and knockdown with retrovirus, results of western blot and Real-time RT-PCR showed that the overexpression and knockdown efficiency of DLX5 was significantly high. ②The overexpression of DLX5 enhanced migration and chemotaxis capacities of SCAPs. ③The knockdown of DLX5 inhibited migration and chemotaxis capacities of SCAPs. ④The DLX5-overexpressing conditioned medium of SCAPs promoted migration and chemotaxis capacities of PDLSCs. Conclusion DLX5 significantly promotes migration and chemotaxis capacities of SCAPs. The DLX5-overexpressing conditioned medium of SCAPs promotes migration and chemotaxis capacities of PDLSCs.

Key words: distal-less homeobox 5, dental tissue-derived mesenchymal stem cell, migration, chemotaxis, conditioned medium

CLC Number: