Claudin-7在口腔鳞状细胞癌中的表达和作用研究

doi:10.13591/j.cnki.kqyx.2022.07.006

Abstract

Abstract: Objective To explore the expression and role of Claudin-7 in oral squamous cell carcinoma (OSCC). Methods Expression of Claudin-7 in OSCC was detected by immunohistochemistry experiment and western blot experiment, and the relationship between Claudin-7 and pathological parameters was analyzed. Lentiviral was used to transfect Cal27 and HN6 cell line to increase the expression of Claudin-7. CCK-8 assay and Transwell assay were used to detect changes in cell proliferation and migration ability. Western blot experiments and RT-qPCR experiments were used to detect the effect of up-regulated Claudin-7 on the index of epithelial-mesenchymal transition. Results Claudin-7 was decreased in OSCC (P<0.05) and its expression level was related to patient′s tumor size, differentiation degree and lymph node metastasis (P<0.05). Overexpression of Claudin-7 could inhibit the proliferation and migration of Cal27 and HN6 cells, up-regulate the expression of E-cadherin protein and mRNA, and down-regulate the expression of N-cadherin and Vimentin protein and mRNA (P<0.05). Conclusion Claudin-7 is expressed at low levels in OSCC. Up-regulating the expression of Claudin-7 can inhibit the proliferation and migration of OSCC cells by regulating epithelial-mesenchymal transition. Claudin-7 can be used as a new target for the treatment of OSCC.

Key words: Claudin-7, oral squamous cell carcinoma, epithelial-mesenchymal transition, proliferation, migration

CLC Number:

R739.8

WANG Jinxin, YI Jie, DING Yujie, ZHONG Yi, SUN Zhida. Expression and role of Claudin-7 in oral squamous cell carcinoma[J]. Stomatology, 2022, 42(7): 604-608.

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Expression and role of Claudin-7 in oral squamous cell carcinoma

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