›› 2016, Vol. 36 ›› Issue (1): 37-39.

• Basic Research • Previous Articles     Next Articles

Ping HE,   

  • Received:2015-06-08 Revised:2015-09-10 Online:2016-01-28 Published:2016-01-08

Abstract: Abstract Objective:To observe the effects of leptin on the proliferation and differentiation of bone marrow mesenchymal stem cells(BMSCs) in vitro. Methods:Sprague Dawley rat derived BMSCs were cultured and located on the surface of titanium disc in the 6-well plate. 50nmol/L leptin was added into the experimental group, compare the difference of the activity of alkaline phosphatase(ALP) and osteocalcin(OC) between the experimental group and the control group, and the proliferative activity was examined by MTT. Results:There were significant differences in the activity of ALP and OC between the experimental and control groups(P<0.05), no difference was found in the cell proliferation. Conclusion:Leptin can stimulate the differentiation of BMSCs on titanium surface in vitro.

Key words: Key words: leptin, BMSCs, osteogenesis