慢性肾脏病继发小鼠颌骨异常的形态学研究

Abstract

Abstract: Abstract：Objective To investigate the morphological characteristics of maxillofacial bone abnormalities secondary to chronic kidney disease (CKD), meanwhile evaluating the biological function of mesenchymal stem cells (MSCs) and osteoclast precursors from the mouse model in vitro. Methods A mouse model of CKD was established by adenine diet induction. Monitor changes in body weight and serum biochemical indicators of blood urea nitrogen (BUN), creatinine (CREA), calcium (Ca), and phosphorus (P) every 4 weeks, and perform HE staining of kidney tissues at the end of the 12th week. The mandibles were analyzed by Micro-CT and HE staining. Enzyme-linked immunosorbent assay (ELISA) was used to determine the serum level of parathyroid hormone (PTH). MSCs were cultured in vitro to detect their proliferation and osteogenic differentiation ability. Bone marrow macrophage (BMMs) were induced into osteoclasts to observe the osteoclast differentiation. Results Compared with the normal control group, the body weights were decreased and serum levels of BUN, CREA, CA, P were increased in the CKD group(P<0.05). The HE staining of kidney tissues in the CKD group showed the number of glomerulus was decreased, renal interstitial inflammatory cell infiltration and fibrosis. Micro-CT analysis revealed that the bone density and mass of mandible were reduced in the CKD group (P<0.05); The HE staining of the CKD group showed an increase in mandibular osteoid and irregular trabecular shape. ELISA showed serum PTH levels were significantly increased in the CKD group (P<0.05). In vitro experiments showed that the proliferation and early osteogenic differentiation ability of MSCs of the CKD group were increased(P<0.05), but CKD osteoblasts mineralized slowly in the later period(P<0.05). The TRAP staining results showed osteoclast differentiation to be more active in the CKD group(P<0.05). Conclusions Hyperparathyroidism secondary to CKD can lead to osteogenesis and mineralization disorder of high-proliferation MSCs in the late stage，and contribute to the differentiation of osteoclast, and accelerate bone transformation. The maxillofacial bone may show a decrease in bone mass and changes in bone microstructure.

Key words: Chronic kidney disease parathyroid hormone bone microstructure Osteoblast Osteoclast

CLC Number:

R783.5

References 20

[1]	Sagliker Y, Balal M, Sagliker Ozkaynak P, et al.Sagliker syndrome: uglifying human face appearance in late and severe secondary hyperparathyroidism in chronic renal failure[J].Seminars in nephrology, 2004, 24(5):449-455
[2]	马丹丹, 董静, 徐宝华.综合征病因的初步研究[J].中国医刊, 2012, 47(10):34-36
[3]	Pontes FSC, Lopes MA, de Souza LL, et al.Oral and maxillofacial manifestations of chronic kidney disease-mineral and bone disorder: a multicenter retrospective study[J].Oral surgery, oral medicine, oral pathology and oral radiology, 2018, 125(1):31-43
[4]	王庆婷，崔颖，李云飞等.调整腺嘌呤饮食磷含量制作慢性肾病高磷血症的小鼠模型[J].南京医科大学学报自然科学版, 2018, 38(07):956-961
[5]	Tani T, Orimo H, Shimizu A, et al.Development of a novel chronic kidney disease mouse model to evaluate the progression of hyperphosphatemia and associated mineral bone disease[J].Scientific reports, 2017, 7(1):2233-
[6]	Jia T, Olauson H, Lindberg K, et al.A novel model of adenine-induced tubulointerstitial nephropathy in mice 2013, 14: 116.[J].BMC nephrology, 2013, 14:116-
[7]	Williams MJ, Sugatani T, Agapova OA, et al.The activin receptor is stimulated in the skeleton,vasculature,heart,and kidney during chronic kidney disease[J].Kidney international, 2018, 93(1):147-158
[8]	杜海明, 张旗.缺失后小鼠下颌骨微结构增龄性变化的-评价[J].口腔颌面外科杂志, 2017, 27(01):13-18
[9]	Hsu PY, Tsai MT, Wang SP, et al.Cortical Bone Morphological and Trabecular Bone Microarchitectural Changes in the Mandible and Femoral Neck of Ovariectomized Rats[J].PloS one, 2016, 11(4):e0154367-
[10]	秦东泽，胡奥，郭威孝等.对不同时间和部位松质骨影响的-分析[J].口腔医学, 2017, 37(03):198-202
[11]	赵航, 盛青，苏俭生.小鼠颌骨间充质干细胞优化获取及鉴定 [J]. 2014, 24 (04):256-260.[J].口腔颌面外科杂志, 2014, 24 (04):256-260
[12]	李胜丹, 梁乙然, 刘一涵.衰老性骨质疏松微环境对颌骨骨髓间充质干细胞生物学功能的影响[J].中华老年口腔医学杂志, 2019, 17(04):198-203
[13]	Kim SH, Kim MO, Kim HJ, et al.Bortezomib prevents ovariectomy-induced osteoporosis in mice by inhibiting osteoclast differentiation[J].Journal of bone and mineral metabolism, 2018, 36(5):537-546
[14]	Ni LH, Tang RN, Lv LL, et al.A rat model of SHPT with bone abnormalities in CKD induced by adenine and a high phosphorus diet[J].Biochemical and biophysical research communications, 2018, 498(3):654-659
[15]	Isakov O, Rinella ES, Olchovsky D, et al.Missense mutation in the MEN1 gene discovered through whole exome sequencing co-segregates with familial hyperparathyroidism[J].Genetics research, 2013, 95(4):114-120
[16]	Jover Cerveró A, Bagán JV, Jiménez Soriano Y, et al.Dental management in renal failure: patients on dialysis[J].Medicina oral, patologia oral y cirugia bucal, 2008, 13(7):E419-426
[17]	Messier MD, Emde K, Stern L, et al.Radiographic periodontal bone loss in chronic kidney disease[J].Journal of periodontology, 2012, 83(5):602-611
[18]	Lee MM, Chu EY.El-Abbadi MM,et alCharacterization of mandibular bone in a mouse model of chronic kidney disease[J].J Periodontol, 2010, 81(2):300-309
[19]	江建青.尿毒症高转化骨病大鼠BM-MSC培养鉴定及增殖分化的信号转导机制 [D]. 天津医科大学, 2013.
[20]	Wu W, Qian LY, Chen XD, et al.A case of Sagliker syndrome and literature review[J].Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2014, 39(10):1100-1104

Study of morphological feature and mechanism of maxillofacial bone abnormalities secondary to chronic kidney disease in mice

Knowledge

Abstract

Cite this article

share this article

References 20

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	. Analysis on buccal bone thickness of mandibular anterior teeth in malocclusion adolescents in different growing periods [J]. , 2020, 40(7): 618-623.
[2]	. The upper airway change of patients treated with Mandibular Molars Distalization by Clear Aligner [J]. , 2020, 40(6): 531-534.
[3]	qihao lu. Correlation between vertical facial type and tooth-alveolar bone complex morphology in maxillary posterior teeth of adult patients with skeletal II [J]. , 2020, 40(5): 432-436.
[4]	Ying-Qian WU. Malocclusion assessment index and its application in early orthodontic treatment [J]. , 2020, 40(5): 476-480.
[5]	. Study of the vertically impacted maxillary incisors’ root development during orthodontic traction [J]. , 2020, 40(4): 330-333.
[6]	SHAO xinyi. The study of orthodontic orthognathic treatment of skeletal class Ⅲ patients by using visual analogue score (VAS) [J]. , 2020, 40(4): 334-337.
[7]	. Comparison of two kinds of micro-implant anchorage methods in the orthodontic treatment with teeth extraction [J]. , 2020, 40(4): 338-341.
[8]	. Study on the experimental conditions of nano-indentation test for investigating the viscoelastic properties of human periodontal ligament [J]. , 2020, 40(4): 313-318.
[9]	. Effects of different types of malocclusions on morphology and function of temporomandibular joint [J]. , 2020, 40(4): 381-384.
[10]	. The Expression of Hedgehog downstream signals in orthodontic bone remodeling. [J]. , 2020, 40(4): 293-298.
[11]	. [J]. , 2020, 40(3): 219-222.
[12]	. Clinical study of two methods for the treatment of Class Ⅱ division 2 of different ages [J]. , 2020, 40(3): 209-214.
[13]	. Research of changes of 61 patients’periodontal condition following orthodontic extrusion in retaining residual root treatment [J]. , 2020, 40(3): 215-218.
[14]	. New progress and Prospect of bisphosphonate in orthodontic treatment [J]. , 2020, 40(3): 266-270.
[15]	. Analysis of the soft and hard tissue changes after orthognathic surgery in patients with facial asymmetry by threedimensional photography combined with conebeam computed tomography scans [J]. , 2020, 40(2): 117-120.