诺丽联合EDTA应用对牙本质机械性能的影响

doi:10.13591/j.cnki.kqyx.2023.08.004

摘要/Abstract

摘要：

目的研究诺丽提取物溶液(Morinda citrifolia juice,MCJ)联合EDTA对牙本质机械性能的影响,评估MCJ作为根管冲洗液应用于根管治疗的效果。方法选取21颗人单根前磨牙和36颗人第三磨牙,根据冲洗液不同随机分为阴性对照组和5个实验组。阴性对照组:蒸馏水;实验组:A组5.25% NaClO、B组6% MCJ、C组17% EDTA、D组5.25% NaClO-17% EDTA、E组6% MCJ-17% EDTA。随机取12颗人单根前磨牙,近远中向劈开,每个半根为一个样本,共24个样本。不同冲洗液浸泡后进行显微硬度测试;剩余的9颗人单根前磨牙,制备36个沙漏型牙本质样本块,浸泡后进行极限拉伸强度测试;36颗人第三磨牙截根后切割,冠部牙本质制备72个矩形牙本质本块,浸泡后每组随机取6个样本分别进行三点弯曲强度和压缩强度测试。结果 ①根颈1/3区,B、E组牙本质显微硬度明显大于A组、C组和D组(P<0.05);根中1/3区,B组牙本质显微硬度明显大于A、C、D、E组(P<0.05);根尖1/3区,B、E组牙本质显微硬度明显大于A组牙本质显微硬度(P<0.05)。②B、D、E组牙本质极限抗拉强度明显高于A、C组(P<0.05)。③B组牙本质三点弯曲强度明显高于A、C组,E组牙本质三点弯曲强度明显高于D组(P<0.05)。④B、E组牙本质压缩强度明显高于A、D组(P<0.05)。结论诺丽对牙本质机械性能的影响小于5.25% NaClO;诺丽联合17% EDTA应用对牙本质机械性能的影响小于5.25% NaClO联合17% EDTA。

关键词: 根管冲洗, 诺丽, 机械性能

Abstract:

Objective To study the effect of Morinda citrifolia juice combined with EDTA on the mechanical properties of dentin, and evaluate the application prospect of MCJ as a root canal irrigation solution. Methods Twenty-one human single-rooted premolars and 36 human third molars were selected and randomly divided into negative control group and five experimental groups according to different immersion protocols. Negative control group: distilled water;experimental group: 5.25% NaClO in group A, 6% MCJ in group B, 17% EDTA in group C, 5.25% NaClO-17% EDTA in group D, and 6% MCJ-17% EDTA in group E. 12 human single-rooted premolars were randomly selected and split in the mesio-distal direction. Each half root was one sample, so there were a total of 24 samples. They were randomly divided into 6 groups, and 4 samples from each group for micro-hardness test. The remaining 9 human single-rooted premolars were split mesio-distally to prepare 36 hourglass dentin sample blocks, and 6 samples were randomly selected from each group for ultimate tensile strength test. After root resection of 36 human third molars, coronal dentin prepared 72 rectangular dentin blocks. After immersion, 6 samples were randomly selected from each group for flexural strength and compressive strength tests, respectively. Results ①In the crown 1/3 area, the dentin micro-hardness in groups B and E were significantly greater than that in groups A, C and D(P<0.05); in the middle 1/3 area of the root, the dentin micro-hardness in group B was significantly greater than that in groups A, C, D, and E(P<0.05);in the apical 1/3 area, the dentin micro-hardness in groups B and E were significantly greater than that in group A(P<0.05). ②The ultimate tensile strength of dentin in groups B, D and E were significantly higher than that in groups A and C(P<0.05). ③The dentin flexural strength of group B was significantly higher than that of groups A and C, and the dentin flexural strength of group E was significantly higher than that of group D(P<0.05). ④The dentin compression strength in groups B and E were significantly higher than that in groups A and D(P<0.05). Conclusion MCJ has less effect on the mechanical properties of dentin than 5.25% NaClO;MCJ combined with 17% EDTA root canal irrigation has less effect on the mechanical properties of dentin than 5.25% NaClO combined with 17% EDTA.

Key words: root canal irrigation, Morinda citrifolia juice, mechanical properties

中图分类号:

R781.05

张岚, 李艳萍, 何丽娜, 潘爽, 王冉旭, 牛玉梅. 诺丽联合EDTA应用对牙本质机械性能的影响[J]. 口腔医学, 2023, 43(8): 690-696.

ZHANG Lan, LI Yanping, HE Lina, PAN Shuang, WANG Ranxu, NIU Yumei. Effects of Morinda citrifolia combined with EDTA root canal irrigation on the mechanical properties of dentin[J]. Stomatology, 2023, 43(8): 690-696.

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组别	根颈1/3/HV_0.1	根中1/3/HV_0.1	根尖1/3/HV_0.1	F	P
(对照组)蒸馏水	66.85±3.08^acde	71.95±4.38^acde	61.95±3.82^ad
(A组)NaClO	47.90±8.48^abd	55.33±4.71^abce	51.65±9.66^abce	1.326	0.295
(B组)MCJ	61.38±4.85^abcd	70.13±2.64^abcde	59.73±3.87^a	12.367	0.001
(C组)EDTA	50.08±4.13^bce	61.95±2.36^abcd	58.71±6.93^ac	9.589	0.002
(D组)NaClO-EDTA	49.62±4.70^bde	54.93±2.36^bcde	54.65±3.39^d	4.107	0.038
(E组)MCJ-EDTA	56.83±1.10^acd	62.08±3.86^abde	59.23±1.53^ae	6.746	0.008
F	14.214	24.778	2.792
P	<0.001	<0.001	0.035

组别	极限拉伸强度/MPa
(对照组)蒸馏水	43.25±10.90^abcde
(A 组)NaClO	8.22±2.38^abcde
(B 组)MCJ	21.97±3.15^abc
(C 组)EDTA	14.12±2.68^abcde
(D 组)NaClO-EDTA	20.00±1.13^acd
(E 组)MCJ-EDTA	24.58±2.01^ace
F	35.022
P	<0.001

组别	弯曲强度/MPa
(对照组)蒸馏水	199.39±6.25^abcde
(A 组)NaClO	96.80±24.55^ab
(B 组)MCJ	138.63±23.77^abcde
(C 组)EDTA	99.43±11.71^bcd
(D 组)NaClO-EDTA	79.48±11.71^bcde
(E 组)MCJ-EDTA	114.16±11.20^bde
F	41.573
P	<0.001

组别	压缩强度/MPa
(对照组)蒸馏水	75.06±0.04^ad
(A 组)NaClO	65.77±7.73^abcde
(B 组)MCJ	74.00±2.56^abd
(C 组)EDTA	74.76±0.67^acd
(D 组)NaClO-EDTA	58.32±8.68^bcde
(E 组)MCJ-EDTA	75.03±0.04^ade
F	12.279
P	<0.001