浓缩生长因子促进大鼠下颌骨联合部成骨作用的初步研究

doi:10.13591/j.cnki.kqyx.2023.11.003

摘要/Abstract

摘要：

目的探讨CGF对促进大鼠下颌骨联合部成骨的作用及机制研究。方法 24只SD大鼠随机分为三组,于大鼠下颌骨联合部缺损处分别填满:单纯Bio-Oss胶原骨(胶原骨组);Bio-Oss 胶原骨并于表面覆盖CGF凝胶(CGF+胶原骨组);单纯CGF凝胶(CGF组)。分组手术第3、6个月后,分两批处死大鼠。离体标本拍摄micro-CT,通过三维重建及截断面比较各组间放射学表现,按标准评分后进行统计学分析。标本拍摄micro-CT完成后脱钙处理,制作组织学切片,观察比较各组间的差异。结果结果表明第3个月时,CGF+胶原骨组评分要明显高于CGF组和胶原骨组(P=0.026 6,P=0.026 6),而CGF组和胶原骨组间差异无统计学意义;第6个月时,CGF+胶原骨组评分与CGF组和胶原骨组相比,具有显著优势(P=0.002 1,P=0.010 1),而胶原骨组评分看似优于CGF组,却差异无统计学意义。通过HE染色发现,CGF+胶原骨组在第3个月时评分显著优于CGF组和胶原骨组(P=0.003 8,P=0.003 8),而CGF组和胶原骨组间差异无统计学意义;第6个月时,与CGF组和胶原骨组相比,CGF+胶原骨组评分依然具有显著优势(P=0.008 2,P=0.019 4),胶原骨组评分略微高于CGF组,然而在统计学分析上无显著差异。结论 CGF与胶原骨联合使用可以明显促进骨缺损的修复,但单独使用CGF对大范围骨缺损的效果并不理想。

关键词: 大鼠下颌骨联合部, 浓缩生长因子, 成骨

Abstract:

Objective To investigate the effect of CGF on promoting the osteogenesis of the mandible symphysis in rats, and to preliminarily explore the possible mechanism. Methods A total of 24 rats were randomly divided into three groups: Collagen group, Collagen + CGF group, CGF group. Three and six months after operation, the rats were killed in two batches. The in vitro specimens were photographed by micro-CT, and the radiological manifestations of each group were compared by three-dimensional reconstruction and cross-section. Statistical analysis was conducted according to standard scores. The specimens were decalcified after micro-CT, and histological sections were made to compare differences among the groups. Results Statistical analysis showed that at the 3rd month, the score of CGF+Collagen group was significantly higher than that of CGF group and Collagen group(P=0.026 6,P=0.026 6), but there was no significant difference between CGF group and Collagen group. At the 6th month, CGF+Collagen group had a significant advantage compared with CGF group and Collagen group(P=0.002 1,P=0.010 1). Collagen group seemed to be better than CGF group, but there was no statistical difference. Through HE staining, it was found that the score of CGF+Collagen group was significantly better than that of CGF group and Collagen group at the 3rd month(P=0.003 8,P=0.003 8), but there was no significant difference between CGF group and Collagen group. At the 6th month, compared with CGF group and Collagen group, the score of CGF+Collagen group was still significantly higher than that of Collagen group (P=0.008 2,P=0.019 4). The score of Collagen group was slightly higher than that of CGF group, but there was no significant difference. Conclusion The combination of CGF and Collagen can significantly promote the healing of bone defects, but the effect of CGF alone on bone defects is not ideal.

Key words: rat mandibular symphysis, concentrated growth factor, osteogenesis

中图分类号:

R782.23

卢鹏, 陈祉宏, 杨月美, 尹忠浩, 吴福丽, 宋晓萌, 吴煜农. 浓缩生长因子促进大鼠下颌骨联合部成骨作用的初步研究[J]. 口腔医学, 2023, 43(11): 975-980.

LU Peng, CHEN Zhihong, YANG Yuemei, YIN Zhonghao, WU Fuli, SONG Xiaomeng, WU Yunong. A preliminary study on the effect of CGF on osteogenesis in the rat mandibular symphysis[J]. Stomatology, 2023, 43(11): 975-980.

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得分值	CGF	CGF+胶原骨	胶原骨
第3个月时
0分	2	0	2
1分	2	1	2
2分	0	3	0
3分	0	0	0
第6个月时
0分	2	0	1
1分	2	0	2
2分	0	1	1
3分	0	3	0

检测时间	CGF	CGF+胶原骨	胶原骨
3个月	0.27±0.05	0.41±0.05	0.35±0.02
6个月	0.29±0.06	0.48±0.03	0.38±0.05

检测时间	CGF	CGF+胶原骨	胶原骨
3个月	(38.28±8.27)%	(63.34±5.79)%	(46.19±5.97)%
6个月	(40.28±9.30)%	(78.28±6.85)%	(51.37±6.40)%