›› 2013, Vol. 33 ›› Issue (2): 84-86.

• 基础与临床研究 • 上一篇    下一篇

细胞色素C在原发性干燥综合征患者唇腺组织中表达的研究

康媛媛,闻妍,张英,孙妍   

  1. 中国医科大学口腔医学院
  • 收稿日期:2012-02-13 修回日期:2012-03-11 出版日期:2013-02-08 发布日期:2013-02-22
  • 通讯作者: 康媛媛 E-mail:kyandly@sina.com
  • 基金资助:
    辽宁省科学技术计划项目 干燥综合征患者唇腺组织中Cytc介导的凋亡信号蛋白的研究

Study on expression of cytochrome C in the labial glands of patients with primary Sjogren’s syndrome

  • Received:2012-02-13 Revised:2012-03-11 Online:2013-02-08 Published:2013-02-22

摘要: 目的 检测原发性干燥综合征患者唇腺组织中CytC的表达水平,探讨CytC在原发性干燥综合征发病中的作用。方法 分别采用免疫组化和逆转录-聚合酶链式方法对40例原发性干燥综合征患者和22例非干燥综合征患者的唇腺组织中CytC蛋白和mRNA进行检测, 比较分析其在两组之间的差异。使用SPSS12.0软件对数据进行t检验。结果 原发性干燥综合征患者组织中CytC蛋白和mRNA的表达明显高于非干燥综合征患者,2组之间具有显著性差异。结论 CytC在原发性干燥综合征患者唇腺组织中存在表达异常。

关键词: 原发性干燥综合征, 唇腺, 细胞色素C, 凋亡, primary Sjogren’s syndrome, labial gland, cytochrome C, apoptosis

Abstract: Abstract: Objective To investigate the expression of CytC in the labial glands of patients with primary Sjogren’s syndrome (pSS) and explore the effects of CytC on pSS. Methods Immunohistochemistry and RT-PCR were used separately to examine CytC protein and mRNA in labial glands in 40 patients with pSS and 22 patients with non- pSS and to make an analysis of their differences. SPSS12.0 software package was used for t test. Results The expression levels of CytC protein and CytCmRNA in 40 patients with pSS were higher than those in 22 patients with non-pSS. (P<0.05). There was significant difference between pSS and non- pSS. Conclusion The study reveals that the CytC expresses abnormally in the labial glands of patients with pSS.

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