关键词: 骨缺损, 种植体, Bio-Oss胶原, 骨组织工程, bone defects, implant, Bio-Oss collagen, bone tissue engineering

Abstract: Objective To evaluate the effects of repairing bone defects around dental implants with tissue engineering bone established with Bio-oss collagen scaffold. Methods Four minipigs were divided into 2 groups randomly. The bone marrow stroma cells (BMSCs) were separated with adherent methods. They were cultured, proliferated and induced in osteogenic induction medium. Collagen I and BGP were evaluated by immunocytochemistry. The induced passage 3 cells and Bio-oss collagen were mixed as a complex. The experimental model was established. The complex was implanted into peri-implant bone defects. Animals were sacrificed at the end of the 8th week and the 16th week. Element Ca2+ in the specimens and its osteogenesis were analyzed with X-ray examination, histological examination, and energy disperse analysis. Results Staining of collagen I and BGP was positive by immunocytochemistry. Von Kossa staining proofed the formation of mineralization nodules. Scanning electron micrographs showed the cells adhered to the inner surfaces of the Bio-oss collagen. At the 8th week, the new bone was more in group BMSCs / Bio-oss collagen than that in group of Bio-oss collagen. There was significant difference between two groups in the percentages of element Ca2+(P<0.05) according to energy disperse anaylsis. At the 16th week, there was good osseointegration in two groups, and there was no significant difference in the percentages of element Ca2+ between two groups according to energy disperse anaylsis. Conclusion Using BMSCs/Bio-oss collagen complex can shorten the time for osseointegration in reparing bone defects around implants in minipigs compared with using Bio-oss collagen alone.