关键词: C/C-SiC复合材料, Caspase-3, bcl-2 , bax , 组织学研究, 日本大耳白兔, C/C-SiC composites, Caspase-3, bcl-2, bax, histology, Japanese bigeared rabbit

Abstract: Abstract: Objective To evaluate the apoptosis of osteocytes to determine whether the C/CSiC composites can be used as implant materials. Methods 8 Japanese bigeared rabbits were selected and randomly divided into group A and group B（n=4）.Implant respecitvely 8 pieces of experimental material（C/CSiC composites） and control material（Ti） into femur of the rabbits.Three months later,decalcify the femur specimens with experimental material and control material and immunohistochemical stain them to observe the combination of interface growth and apoptosis of bone tissue.Use Imagepro Plus 6.0 image analysis software to analyse the gray values of brownstained cells,and use SPSS 17.0 statistical software to make ttest analysis of independent samples. Results The experimental animals were anesthetized well and the effect of surgery was good.Implant materials and bone combined tightly.No loosening happened.New bone grew on most of the surfaces of implant materials.Under the light microscopy,cytoplasm of osteocytes was product of immunohistochemistrical positive reaction of caspase3,Bcl2 and Bax,and brown staining of some cell membrane was observed.There was no significant difference in mean grey value between the pure titanium control group and C/CSiC composite experimental group. Conclusions C/CSiC composites and titanium material were implanted in rabbit femurs.3 months later,the apoptosis of the osteoblasts around materials had no significant difference.