间歇性完全鼻阻塞对幼年大鼠髁突软骨细胞凋亡影响的研究

›› 2014, Vol. 34 ›› Issue (7): 492-496.

间歇性完全鼻阻塞对幼年大鼠髁突软骨细胞凋亡影响的研究

刘炯¹,陈金东¹,朱敏²,聂萍¹,盛潇¹,赵彦惠¹,徐晓珑¹,陶丽¹

1. 上海市黄浦区制造局路639号一号楼405
2. 上海市第九人民医院正颌正畸科

收稿日期:2013-08-05 修回日期:2013-09-17 出版日期:2014-07-28 发布日期:2014-08-04
通讯作者: 朱敏 E-mail:zminnie@126.com
基金资助:
上海市科委课题

Study on influence of bilateral intermittent nasal obstruction on condylar cartilage cell apoptosis in young rats

Received:2013-08-05 Revised:2013-09-17 Online:2014-07-28 Published:2014-08-04
Contact: Min Zhu E-mail:zminnie@126.com

摘要/Abstract

摘要： 目的研究幼年大鼠在双侧间歇性鼻阻塞情况下双侧髁突软骨细胞的凋亡情况，来尝试探讨间歇性鼻阻塞对张口呼吸儿童髁突软骨发育的影响。方法将20只4周龄大鼠（Sprague-Dawley rat,SD rat）分为2组，A组：双侧鼻阻塞组，常氧条件下鼻孔双侧阻塞8 h（8.00AM-16.00PM），持续35 d；B组：对照组 ,常氧条件下饲养。2组给予同样的饮食以及饮水，然后分别取出双侧髁突，用甲醛保存做成石蜡切片，同时进行caspase-3以及Bcl-2，Bax蛋白免疫组化染色。以观察在双侧鼻阻塞对髁突前斜面中上部软骨细胞增殖以及凋亡的影响，以及双侧间歇性鼻阻塞情况下Bcl-2基因家族的调控机制以及与下颌骨生长发育的关系。结果在间歇性双侧完全鼻阻塞情况下，SD幼年大鼠双侧髁突软骨细胞凋亡较对照组明显增加。结论双侧鼻阻塞的未成年大鼠前斜面中上部髁突细胞Bcl-2蛋白以及Bax蛋白，Caspase-3， Bax蛋白表达均较对照组增加，Bcl-2蛋白表达数量实验组较对照组表达减少，髁突软骨细胞凋亡数量较对照组明显增加。

关键词: 双侧间歇性完全鼻阻塞, 下颌髁突, 凋亡

Abstract: Objective To investigate the condylar cartilage cell apoptosis in young rats with bilateral intermittent nasal obstruction to try exploring the influence of intermittent nasal obstruction on the development of condylar cartilage of children who have to breathe through mouth. Methods Twenty 4-week-old SD rats were employed and divided into two groups. Group A：10 of them had both nostrils occluded by nose plugs on normal oxygen conditions up to 8 hours for 35d and group B served as the control group：10 of them were raised under normal oxygen conditions. The same food and water were offered to the two groups and then the bilateral condylar was extracted to make parafin section for the immumohistochemical staining, including Caspase-3, Bcl-2, Bax protein. Cartilage cell proliferation and apoptosis, the regulatory mechanism of Bcl-2 gene family and its correlation with maxillary growth were observed and analyzed. Results Under the circumstance of bilateral intermittent nasal obstruction, the apoptosis of condylar chondrocytes in Group A increased significantly compared with the control group. The expression of caspase-3, bcl-2, bax protein was significantly higher than the control group. Conclusion Oral breathing caused by nasal obstruction results in the apoptosis in the condyle process cells

Key words: nasal obstruction, condyle process, apoptosis

中图分类号:

R349.5

刘炯陈金东朱敏聂萍盛潇赵彦惠徐晓珑陶丽. 间歇性完全鼻阻塞对幼年大鼠髁突软骨细胞凋亡影响的研究[J]. 口腔医学, 2014, 34(7): 492-496.

参考文献

[1]Howard CC.Inherent growth and its influence on malocclusion[J].JADA,1932,19(6):642-651
[2]McNamara JA.Influence of respiratory pattern on craniofacial growth[J].Angle Orthod,1981,51(4):269-300
[3]Monahan R，Seder K,Patel P，et al.Hemifacial microsomia Etiology， diagnosis and treatment[J].J Am Dent Assoc,2001,132(10):1402-1408
[4]Provot S，Schipani E.Molecular mechanisms of endochondral bone development[J].Biochemical and Biophysical Research Communucations,2005,328(3):658-665
[5]Von den Hoff JW,Delatte M.Interplay of mechanical loading and growth factors in the mandibular condyle[J].Arch Oral Biol,2008,53(8):709-715
[6]Galanti C,Musumeci G,Valentino J,et al.A role for apoptosis in temporomandibularjoint disc degenerationA contemporary review[J].. Ital J Anat Embryol,2013,118(1):151-158
[7]Matsuda S，Mishima K，Yoshimura Y，et al.Apoptosis in the development of the temporomandibular joint[J].Anat Embryol （Berl,1997,196(5):383-
[8]Hashimoto S，Setareh M，Roseen F，et al.Fas/FasL Iigand expression and Induction of apoptosis in chondroeytes[J].Arthritis Rheum,1997,40(10):1749-1755
[9]Xu B,buLfone-paus,Aoyama K,et al.Role of fas/fas ligand-mediated Apoptosis in murine contact hypersensitivity[J].Int ImmunoPharmacol,2003,3(7):927-938
[10]Zeuner A,Pedini F,Signore M,et al.Stem cell factor protects erythroid precursor cells from chemotherapeutic agents via up-regulation of BCL-2 family proteins[J].BLOOD,2003,102(1):87-89
[11]Wolter KG,Aiello A,Soligo D,et al.Movement of Bax from the cytosol to mitochondria during apoptosis[J].cell Biol,1997,139(5):1281-1292
[12]Shen G,Darendeliler MA.The adaptive remodeling of condylar cartilage-a transition from chondrogenesis to osteogenesis[J].J Dent Res,2005,84(8):691-699
[13]Wang L，Lazebnik M，Detamore MS.Hyaline cartilage cells outperform mandibular condyalr cartilage cells in a TMJ fibrocartilage t3 engineering application[J].Osteoarthritis Cartilage,2009,17(3):346-353
[14]Jiao K，Wang MQ，Niu LN，et al.Death and proliferation of chondrocytes in the degraded mandibular condylar cartilage of rats induced by experimentally created disordered occlusion[J].Apoptosis,2009,14(1):22-30
[15]Jiao K，Dai J，Wang MQ，et al.Age-and sex-related changes of mandibular condylar cartilage and subchondral bone: a histomorphometric and micro-CT study in rats[J].Arch Oral Biol,2010,55(2):155-163
[16]Matsuda S，Mishima K，Yoshimura Y，et al.Apoptosis in the development of the temporomandibular joint[J].Anat Embryol,1997,196(5):383-391