关键词: 氟斑牙, 氧化应激, 动物模型, 氟中毒

Abstract: Abstract： Objective To learn the effects of oxidative stress on the rat with dental fluorosis caused by coal burning. Methods　High fluoride(F—) air modle was established by burning the fuel,clay rich of F—mixed with coal for 10h a day. Rats were fed with drying corn with different proportions. Thirty SD rats were randomly divided into 5 groups: the high F— air and fed F— 40mg/kg group(H),the high F— air and fed F— 25mg/kg group(M),the high F— air and fed F— 10mg/kg group(L), the high F— air and common food group(A) and the control group(C). All rats were killed at the end of 16th week, and then the activities of SOD, CAT, GSH and MDA levels were measured with their reagent boxes respectively. Results　The dental fluorosis in rats was replicated successfully. The MDA level of the experimental group increased (P<0.01 or P<0.05), while SOD and CAT activities decreased (P<0.01 or P<0.05).No statistically considerable differences was found in GSH level (P>0.05).SOD activities of liver and kidney decreased only in group A(P<0.05). Conclusions　The balance of oxidative and anti-oxidative system may be broken in the rat because of the high F— air. In the occurrence process of coal-burning source dental fluorosis, the oxidative stress of liver and kidney tissue may be earlier than the dental hard-tissue damage caused by high F— air.Oxidative stress may be an important mechanism for body damage and the occurrence of dental fluorosis.

Key words: Dental fluorosis, oxidative stress, animal model, fluorosis