磷酸钙/聚乳酸羟基乙酸复合骨水泥对破骨前体细胞炎症因子表达的影响

›› 2016, Vol. 36 ›› Issue (4): 289-293.

• 基础研究 • 下一篇

磷酸钙/聚乳酸羟基乙酸复合骨水泥对破骨前体细胞炎症因子表达的影响

陈娟娟¹,梁翠薇²,叶财敏²,覃媛²,吴修团¹,谢柳容¹,李文良¹,廖红兵¹

1. 广西医科大学附属口腔医院
2. 广西医科大学附属口腔医院修复科

收稿日期:2015-11-10 修回日期:2016-01-08 出版日期:2016-04-28 发布日期:2016-04-24
通讯作者: 廖红兵 E-mail:liao.hongbing@gmail.com
基金资助:
国家自然科学基金资助项目

Effects of calcium phosphate/poly (lactic-co-glycolic acid) composite cement on expression of inflammation factors in osteoclast precursorsoclast Precursors

Received:2015-11-10 Revised:2016-01-08 Online:2016-04-28 Published:2016-04-24

摘要/Abstract

摘要： 目的观察磷酸钙/聚乳酸羟基乙酸复合骨水泥（CPC/PLGA）对破骨前体细胞炎症因子TNF-α、IL-1、IL-6表达的影响，了解PLGA促进CPC降解的机理。方法 ①根据高效液相色谱法检测出的乳酸、羟基乙酸浓度配置CPC/PLGA替代液。②将RAW264.7细胞分别培养在DMEM培养基（对照组），第6小时CPC初凝块浸提液（A组）及CPC/PLGA替代液（B组）。通过CCK-8法检测各组细胞的增殖变化，并通过RT-PCR法检测各组中TNF-α、IL-1、IL-6 mRNA的表达情况。结果增殖实验结果示，A组细胞的增殖率在5 d时达到最高值（1.01±0.02），而B组细胞的增殖率在5 d时达到最高值（1.07±0.02），均与对照组相比有统计学意义（P＜0.05）。RT-PCR法示A组和B组细胞TNF-α mRNA、IL-1 mRNA在6 h时表达最高，IL-6 mRNA在12 h时表达最高，与对照组比较均有统计学差异(P＜0.05)。结论磷酸钙/聚乳酸羟基乙酸复合骨水泥调控破骨前体细胞炎症因子增高。

关键词: 磷酸钙骨水泥, 聚乳酸-羟基乙酸, 破骨前体细胞, 炎症因子

Abstract: Objective To investigate the effects of calcium phosphate/poly (lactic-co-glycolic acid) composite cement (CPC/PLGA) on expression of inflammation factors TNF-α, IL-1, IL-6 in osteoclast precursors, and to understand the mechanism of PLGA in promoting the degrading of CPC. Methods ① According to the concentration of lactic acid and glycolic acid detected by high performance liquid chromatography (HPLC) method, CPC/PLGA alternative solution was configurated. ② RAW264.7 cells were cultured in DMEM medium (control group), 6 h CPC clot soaking liquid (group A) and CPC/PLGA alternative liquid (group B).The proliferation of RAW264.7 cells were tested by CCK-8 method. Then the cells from each group were stimulated, and subsequently the mRNA expressions of TNF-α, IL-1, IL-6 were analyzed through RT-PCR. Results Proliferation assay revealed that the cell proliferation of group A reached its maximum which was 1.01±0.02 at 5 d, while group B at 5 d was 1.07±0.02, and compared to control group, the differences were statistically significant (P＜0.05); RT-PCR showed that TNF-α mRNA, IL-1mRNA expression in 6 h was highest in group A and group B, while IL-6 mRNA expression in 12 h was highest, and compared with the control group the differences were statistically significant (P＜0.05). Conclusion CPC/PLGA can increase the expression of inflammation factors in osteoclast precursors.

Key words: calcium phosphate cement, poly(lactic-co-glycolic acid), osteoclast precursors, inflammatory factor

中图分类号:

R782.4

陈娟娟梁翠薇叶财敏覃媛吴修团谢柳容李文良廖红兵. 磷酸钙/聚乳酸羟基乙酸复合骨水泥对破骨前体细胞炎症因子表达的影响[J]. , 2016, 36(4): 289-293.

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磷酸钙/聚乳酸羟基乙酸复合骨水泥对破骨前体细胞炎症因子表达的影响

Effects of calcium phosphate/poly (lactic-co-glycolic acid) composite cement on expression of inflammation factors in osteoclast precursorsoclast Precursors

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