碱热处理联合紫外光照后钛体外活性的研究

›› 2017, Vol. 37 ›› Issue (5): 408-412.

碱热处理联合紫外光照后钛体外活性的研究

韩石磊¹,赵颖²,李大鹏²,王羽泽¹,杜青³,谢伟丽¹

1. 哈尔滨医科大学附属口腔医院
2. 哈尔滨医科大学口腔医学院修复科
3. 哈尔滨工业大学

收稿日期:2016-11-30 修回日期:2016-12-29 出版日期:2017-05-28 发布日期:2017-05-19
通讯作者: 谢伟丽 E-mail:xwl811@126.com
基金资助:
黑龙江省科技攻关项目

Effect of alkali-and-heat treatment combined with ultraviolet irradiation on bioactivity of titanium in vitro

Received:2016-11-30 Revised:2016-12-29 Online:2017-05-28 Published:2017-05-19

摘要/Abstract

摘要： 目的研究碱热处理加紫外光照后钛的微观结构变化及在模拟体液中诱导羟基磷灰石沉积的能力。方法将纯钛分组处理：SLA组：喷砂酸蚀；AAH组：SLA组加碱热处理；UV组：AAH组加紫外线辐照处理。测试各组理化性质，对其进行模拟体液浸泡实验。采用扫描电镜及X射线衍射仪观察分析沉积物。结果碱热处理加紫外光照的钛具有多级孔洞的微观粗糙结构，同时引入钙、钠元素，并有最佳的表面润湿性；模拟体液浸泡后，试样表面矿化物质量最多，沉积速度最快，X射线衍射仪分析沉积物为羟基磷灰石。结论碱热处理加紫外光照后纯钛种植体具有良好的体外生物活性。

关键词: 钛种植体, 表面改性, 紫外光辐照, 碱热处理, 模拟体液

Abstract: Objective To study the effect of alkali-and-heat treatment combined with ultraviolet irradiation on properties of titanium surface and the ability of inducing hydroxyapatite(HA) deposition. Methods The specimens were divided into three groups, which were treated by sandblast and acid-etch(SLA),SLA with alkali-and-heat treatment(AAH), AAH with ultraviolet irradiation(UV). The physicochemical properties of these titanium were tested. HA deposit was examined by SEM with EDX and X-ray diffraction(XRD) after simulated body fluid(SBF) immersion test. Results Rough morphology with multilevel holes was formed on titanium after acid-alkali-heat treatment and ultraviolet irradiation. UV showed the best water contact angle,approximately 0°. Besides,calcium and sodium were induced in UV group. HA deposit was found on the specimen surfaces after SBF immersion test with the largest amount and fastest speed. Conclusions The titanium after alkali-and-heat treatment combined with ultraviolet irradiation has suitable bioactivity in vitro.

Key words: titanium implant, surface modification, ultraviolet irradiation, alkali-and-heat treatment, simulated body fluid

中图分类号:

R783.1

韩石磊赵颖李大鹏王羽泽杜青谢伟丽. 碱热处理联合紫外光照后钛体外活性的研究[J]. , 2017, 37(5): 408-412.

参考文献

王立军，郭志君，胡盼，等.不同碱热处理方法对钛表面理化性能及细胞响应特性的影响[J].生物骨科材料与临床研究, 2014, 11(2):5-9. [2]　张悦，夏海斌.碱热处理制备生物活性钛种植体[J].国际口腔医学杂志, 2007, 34(3):216-219. [3]　Aita H,Hori N,Takeuchi M,et al.The effect of ultraviolet functionalization of titanium on integration with bone[J].Biomaterials, 2009, 30(1):1015-25. [4]　卢世松，李玉海，蔡倩.纯钛牙齿种植体生物活性的研究[J].沈阳理工大学学报, 2014, 33(4):14-20. [5]　Jeong SJ,Wang G,Choi BD,et al.Secretory Leukocyte Protease Inhibitor (SLPI) Increases Focal Adhesion in MC3T3 Osteoblast on Titanium Surface[J]．J Nanosci Nanotechnol,2015,15(1):200-4. [6]　Hou J,Li B,Lu S,et al.Regulation of osteoblast proliferation and differentiation by interrod spacing of Sr-HA nanorods on microporous titania coatings.ACS applied materials & interfaces,2013,5(11):5358-5365. [7]　Iezzi G,Degidi M,Piattelli A,et al.A histological and histomorphometrical evaluation of retrieved human implants with a wettable,highly hydrophilic,hierarchically microstructured surface:a retrospective analysis of 14 implants[J].Implant Dent,2013,22(2):138-142. [8]　刘滋川,王芳,郭彬,等.不同钛涂层表面结构对于成骨细胞增殖及分化的影响[J].现代口腔医学杂志, 2015, 29(5):279-282. [9]　Shibata Y, Tanimoto Y, Maruyama N, Nagakura M. A review of improved fixation methods for dental implants.Part II: biomechanical integrity at bone-implant interface. J Prosthodont Res.2015. 59(2): 84-95. [10]　Kokubo T,Takadama H.How useful is SBF in predicting in vivo bone bioactivity?[J].Biomaterials, 2006,27(15):2907-2915. [11]　田智慧，张宇，王立超，等.微弧氧化处理纯钛种植体表面在模拟体液中可诱导磷灰石沉积[J].南方医科大学学报, 2013, 33(10):1554-1556. [12]　王小红，曹阳，张利，等.碱热处理对钛表面生物活性的影响[J].功能材料, 2013, 44(2):275-280.

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[3]	李智葛少华. 纳米二氧化钛在生物医学中的应用进展[J]. , 2017, 37(1): 85-88.
[4]	杜月明周雪峰章非敏刘梅. 表面接枝技术对纤维加强树脂基桩力学性能的影响[J]. , 2016, 36(8): 673-676.
[5]	杜义军董福生栗兴超张雪敏. 匹配牙槽窝的根形种植体即刻种植的动物实验研究[J]. , 2015, 35(9): 721-725.
[6]	黄丽娟,李建,景双林,韩霖霏. 两种表面改性方法对钴-铬合金化学腐蚀行为的影响[J]. , 2009, 29(5): 246-247，268.
[7]	何志良,王银龙,程继光,唐慧,孙麟,谢红军,朱坤鹍,黄擎,陈乔尔. 两焦点与三焦点牵张成骨方式在下颌骨缺损修复中的应用[J]. , 2008, 28(4): 177-179.
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