次氯酸钠和EDTA预处理对釉质白斑树脂粘接强度影响的实验研究

doi:10.13591/j.cnki.kqyx.2025.03.005

摘要/Abstract

摘要：

目的比较次氯酸钠与EDTA预处理对釉质白斑表面釉质孔隙暴露率、树脂渗透效果及其与树脂粘接强度的影响。方法本实验选取完整离体前磨牙共104颗,其中64颗用于制备颊侧中央4 mm×4 mm×2 mm大小的釉质块样本。随后,将样本进行分组:SE组(正常釉质对照组)、DE组(脱矿处理组)、RI组(树脂渗透处理组)、N/RI组(经5.25%NaClO预处理后树脂渗透组)以及E/RI组(经17%EDTA预处理后树脂渗透组)。实验采用扫描电镜技术和激光共聚焦显微镜技术,对各组样本的釉质孔隙暴露率及树脂渗透深度进行精确观察和测量。另外,对剩余的40颗离体牙进行截根处理,将牙冠包埋在树脂块中(仅暴露颊侧釉质面),根据上述分组信息进行相应处理并在颊侧釉质面中央粘接圆柱形树脂柱,经剪切实验记录每组样本的剪切粘接强度及断裂模式。结果与RI组相比,N/RI组和E/RI组样本的釉质表面孔隙暴露率、树脂渗透深度和树脂渗透面积百分比等均显著提高(P<0.05)。SE组剪切粘接强度最高,DE组最低;与DE组相比,RI组、N/RI组、E/RI组样本的剪切粘接强度明显提高(P<0.05)。结论 5.25%NaClO或17%EDTA预处理可有效改善釉质孔隙暴露,改善树脂渗透效果,提高釉质白斑与树脂的粘接强度。

关键词: 釉质白斑, 树脂渗透, 剪切粘接强度, 次氯酸钠, EDTA

Abstract:

Objective To compare the impact of NaClO and EDTA pretreatment on the enamel surfaces pore exposure rate, resin infiltration effectiveness and shear bond strength between the enamel white spot lesions (WSLs) and resin composite. Methods A total of 104 sound premolars were selected for the study. 4 mm×4 mm×2 mm enamel blocks from 64 sound premolars were cut in the center of the buccal surfaces of the teeth. All blocks were randomly divided into five groups: group SE(sound enamels as negative control); group DE(enamel blocks demineralised as positive control); group RI(resin infiltration); group N/RI(5.25% NaClO pretreatment and resin infiltration) and group E/RI(17% EDTA pretreatment and resin infiltration). The pore exposure rate of enamel surfaces and resin penetration depths were observed by scanning electron microscopy(SEM) and confocallaser scanning microscopy(CLSM) in each group. The remaining 40 premolars were cut from the cervical roots and the residual crowns were embedded in self-condensing resin material(buccal surfaces of the enamel exposed only). According to the grouping information, the samples were given the corresponding pretreatments and resin infiltration respectively and were bonded with a cylindrical resin cylinder on the buccal enamel surface for shear bond test. The shear bond strength and fracture modes were recorded and analyzed in each group. Results Compared to group RI, the pore exposure rate of the enamel surface, resin penetration depths, and percentage of resin penetration area in the N/RI and E/RI groups were significantly increased(P<0.05). The shear bond strength of the samples in group SE was the highest, and the lowest in group DE. Compared to group DE, the shear bond strength in groups RI, N/RI, and E/RI was significantly increased(P<0.05). Conclusion Enamel surface pretreatment with 5.25% NaClO or 17% EDTA has been demonstrated to effectively enhance the enamel pore exposure rate, resin penetration depth and the shear bond strength between WSLs and resin composite.

Key words: white spot lesions, resin infiltration, shear bond strength, NaClO, EDTA

中图分类号:

R783.1

贺晶宇, 丁瑜, 杨媛媛, 倪恪, 王琰, 孟箭, 孟庆飞. 次氯酸钠和EDTA预处理对釉质白斑树脂粘接强度影响的实验研究[J]. 口腔医学, 2025, 45(3): 185-190.

HE Jingyu, DING Yu, YANG Yuanyuan, NI Ke, WANG Yan, MENG Jian, MENG Qingfei. The effect of NaClO and EDTA pretreatment on the shear bond strength between the enamel white spot lesions and resin composite: An in vitro study[J]. Stomatology, 2025, 45(3): 185-190.

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组别	釉质表面孔隙暴露面积/μm²	釉质表面孔隙暴露率/%
RI组	185.04±38.66^b	12.90±3.51^b
N/RI组	320.49±56.22^a	20.94±4.74^a
E/RI组	289.12±58.23^a	18.57±3.63^a

组别	LD/μm	PD/μm	PA/%
RI组	173.06±10.62^a	72.13±8.67^b	41.40±5.61^b
N/RI组	173.64±7.89^a	82.14±7.54^a	48.60±4.66^a
E/RI组	172.56±6.49^a	76.83±5.50^ab	45.92±4.56^ab

组别	剪切粘接强度/MPa
SE组	29.70±5.24^a
DE组	20.32±3.97^c
RI组	25.60±3.95^b
N/RI组	26.70±2.67^ab
E/RI组	28.05±3.25^ab