GsMTx4对大鼠牙齿移动过程中痛觉的抑制作用

doi:10.13591/j.cnki.kqyx.2024.11.003

摘要/Abstract

摘要：

目的通过旷场实验、免疫组化染色,研究GsMTx4在牙齿移动过程中对大鼠痛觉的抑制作用。方法选取58只5~6周雄性SD大鼠,并构建右上颌牙齿移动模型。随机将大鼠分为A组(对照组)、B组(GsMTx4组)两组,另按加力时间4 h和1、3、5、7 d分为5个亚组,以A组大鼠左上颌作为C组(空白对照组)。A、B两组大鼠分别局部注射生理盐水、GsMTx4。通过旷场实验评价大鼠的疼痛程度,免疫组化染色观察牙周组织中神经生长因子(nerve growth factor,NGF)、降钙素基因相关肽(calcitonin gene-related peptide,CGRP)的表达情况。结果 4 h、1 d、3 d时,B组大鼠在旷场中5 min内的总路程较A组更高(P<0.05)。在4 h、1 d时,B组大鼠中心区域停留时间百分比较A组高(P<0.05)。A、B两组中NGF、CGRP的表达在1、3、5、7 d时高于C组(P<0.05)。B组NGF、CGRP表达在4 h、1 d、3 d的均低于A组(P<0.05)。结论 GsMTx4在牙齿移动过程中能抑制NGF、CGRP的表达,并缓解牙齿移动时产生的疼痛。

关键词: GsMTx4, 正畸, 疼痛, 神经生长因子, 降钙素基因相关肽

Abstract:

Objective To investigate the inhibitory effect of GsMTx4 on pain sensation in rats during tooth movement, open field experiments and immunohistochemical staining by conducting. Methods Fifty-eight male SD rats aged 5-6 weeks were selected and a right maxillary tooth movement model was constructed. The rats were randomly divided into two groups: group A(control group) and group B(GsMTx4 group). They were also divided into 5 subgroups according to 4 h, 1 d, 3 d, 5 d, and 7 d. The left maxillary of the rats in group A was used as group C(blank control group). The open-field test was used to evaluate the pain level of rats. Immunohistochemical staining was used to observe the expression of nerve growth factor(NGF) and calcitonin gene-related peptide(CGRP) in periodontal tissues. Results At 4 h, 1 d, and 3 d, the total distance traveled by rats in group B within 5 minutes in the open-field was higher than that of group A (P<0.05). At 4 h and 1 d, the percentage of time in the center of rats in group B was higher than that in group A(P<0.05). The expressions of NGF and CGRP in groups A and B were higher than those in group C on 1 d, 3 d, 5 d, and 7 d(P<0.05). The expression of NGF and CGRP in group B were lower than those in group A at 4 h, 1 d, and 3 d(P<0.05). Conclusion GsMTx4 can inhibit the expression of NGF and CGRP during tooth movement and relieve the pain caused by tooth movement.

Key words: GsMTx4, orthodontic, pain, NGF, CGRP

中图分类号:

R783.5

王若飞, 邵丽鑫, 刘晓彤, 张苗苗. GsMTx4对大鼠牙齿移动过程中痛觉的抑制作用[J]. 口腔医学, 2024, 44(11): 815-819.

WANG Ruofei, SHAO Lixin, LIU Xiaotong, ZHANG Miaomiao. The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats[J]. Stomatology, 2024, 44(11): 815-819.

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组别	4 h	1 d	3 d	5 d	7 d
A组	0.110 9±0.010 7^*	0.132 0±0.017 6^*	0.154 0±0.027 3^*	0.129 9±0.024 0^*	0.126 1±0.020 5^*
B组	0.087 2±0.021 2^*#	0.109 3±0.100 9^*#	0.124 2±0.015 0^*#	0.129 7±0.016 9^*	0.117 9±0.015 8^*
C组	0.048 3±0.188 3	0.048 3±0.188 3	0.048 3±0.188 3	0.048 3±0.188 3	0.048 3±0.188 3

组别	4 h	1 d	3 d	5 d	7 d
A组	0.115 8±0.157 2^*	0.164 6±0.017 9^*	0.187 3±0.028 3^*	0.130 4±0.017 4^*	0.130 0±0.016 3^*
B组	0.084 9±0.015 9^#	0.118 0±0.011 9^*#	0.149 8±0.022 5^*#	0.115 1±0.010 6^*#	0.117 9±0.011 8^*
C组	0.094 8±0.014 4	0.094 8±0.014 4	0.094 8±0.014 4	0.094 8±0.014 4	0.094 8±0.014 4