LAPTM5表达与巨噬细胞浸润在牙周炎进展中的相关性研究

doi:10.13591/j.cnki.kqyx.2026.07.003

摘要/Abstract

摘要：

目的探究溶酶体跨膜蛋白5(lysosomal protein transmembrane 5, LAPTM5)在牙周炎组织中的表达变化,分析其与巨噬细胞浸润水平的相关性,并探讨该相关性在牙周炎发生发展中的临床意义。方法整合GEO数据集(GSE16134、GSE171213),通过差异基因分析、功能富集、免疫浸润解析及WGCNA筛选核心基因,结合单细胞数据定位其细胞来源。收集53例牙周炎及5例健康牙龈组织,经免疫组织化学染色验证目标基因与巨噬细胞浸润及炎症的相关性。结果白细胞迁移通路在牙周炎组中富集最显著(P=7.27×10^-6),其活化程度与促炎免疫细胞浸润呈正相关。LAPTM5被鉴定为该通路核心基因,在牙周炎组织中表达上调且与疾病严重程度正相关,单细胞转录组分析及免疫荧光进一步证实LAPTM5定位于巨噬细胞。临床验证显示,CD68⁺巨噬细胞比例与IL-6(P<0.001)及LAPTM5表达水平(P<0.001)均呈显著正相关,提示LAPTM5表达水平与牙周炎严重程度正相关。结论 LAPTM5作为白细胞迁移通路核心基因,可能通过调控巨噬细胞功能参与牙周炎进展,是潜在免疫治疗靶点。

关键词: 牙周炎, 白细胞迁移通路, LAPTM5, 巨噬细胞浸润

Abstract:

Objective To investigate the expression changes of the lysosomal protein transmembrane 5(LAPTM5) in periodontitis tissues, analyze its correlation with the level of macrophage infiltration, and explore the clinical significance of this correlation in the occurrence and development of periodontitis. Methods GEO datasets(GSE16134, GSE171213) were integrated for differential gene expression analysis, functional enrichment, immune infiltration profiling, and WGCNA to identify core genes, combined with single-cell data for cellular localization. Gingival tissue samples from 53 periodontitis patients and 5 healthy controls were collected, and immunohistochemistry was performed to validate the correlation between the target gene and macrophage infiltration as well as inflammation. Results The leukocyte migration pathway was most significantly enriched in periodontitis group (P=7.27×10^-6), and its activity was positively correlated with pro-inflammatory immune cell infiltration. LAPTM5 was identified as the core gene of this pathway, showing upregulated expression in periodontitis tissues, and positively correlated with disease severity. Single-cell RNA sequencing and immunofluorescence analyses confirmed the localization of LAPTM5 in macrophages. Clinical validation revealed that the proportion of CD68⁺ macrophages was significantly positively correlated with IL-6(P<0.001) and LAPTM5(P<0.001) expression levels, suggesting that LAPTM5 expression level is positively correlated with the severity of periodontitis. Conclusion As a core gene of the leukocyte migration pathway, LAPTM5 may participate in periodontitis progression by regulating macrophage function and represents a potential immunotherapeutic target.

Key words: periodontitis, leukocyte migration pathway, LAPTM5, macrophage infiltration

中图分类号:

R781.4

戴书玥, 张茜雅, 陈健, 张沁宇, 周奕一, 毕梦霏, 沈铭. LAPTM5表达与巨噬细胞浸润在牙周炎进展中的相关性研究[J]. 口腔医学, 2026, 46(7): 495-503.

DAI Shuyue, ZHANG Xiya, CHEN Jian, ZHANG Qinyu, ZHOU Yiyi, BI Mengfei, SHEN Ming. Correlation between LAPTM5 expression and macrophage infiltration in the progression of periodontitis[J]. Stomatology, 2026, 46(7): 495-503.

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