关键词: 牙槽骨, 牙周炎, 拉曼光谱, 骨成分

Abstract:

Objective To analyze the compositional characteristics of human alveolar bone in periodontal health and periodontitis at the mid-root and apical regions using Raman spectroscopy, and to investigate differences in inorganic mineral components and collagen secondary structure. Methods Right maxillary central incisors and surrounding alveolar bone samples were obtained from cadaveric jaws with periodontal health or periodontitis. The samples were divided into mid-root and apical regions. Raman spectra were collected from alveolar bone within approximately 1 mm of the tooth root. Quantitative analyses of inorganic mineral parameters and collagen secondary structure were performed. Results In healthy alveolar bone, the mineral-to-matrix ratio in the mid-root region was higher than that in the apical region and was also higher than the mineral-to-matrix ratio in the mid-root region of periodontitis alveolar bone. In periodontitis alveolar bone, the mineral-to-matrix ratios of the mid-root and apical regions were similar. No significant differences were observed in carbonate substitution or bone crystallinity among different alveolar bones or regions. Amide Ⅰ band analysis showed a decreased relative content of α-helix structures and increased relative contents of random coil and β-sheet structures in periodontitis alveolar bone, with more pronounced changes in the mid-root region. The intensity ratio of fitted amide Ⅲ band peak areas(I_{1 245}/I_{1 270}) in the mid-root region of periodontitis alveolar bone was higher than that in the apical region. Conclusion Raman spectroscopy reveals alterations in alveolar bone mineralization and collagen secondary structure under periodontal health and periodontitis, with compositional differences among root regions.

Key words: alveolar bone, periodontitis, Raman spectroscopy, bone composition