大鼠牙齿移动过程中Piezo1对压力侧牙周组织中RIP3及MLKL的影响

doi:10.13591/j.cnki.kqyx.2022.10.004

Abstract

Abstract: Objective To observe the influence of Piezo1 on the necroptosis markers receptor-interacting protein 3 (RIP3) and mixed lineage kinase domain-like protein (MLKL) in pressure-side periodontal tissue at various time points during tooth movement in rats. Methods Seventy-five male SD rats were randomly distributed into control group (group A), force group (group B) and force + inhibitor group (group C). The orthodontic model of the maxillary first molar was constructed in rats. The moving distance of the orthodontic tooth was measured with vernier calipers. Hematoxylin-eosin (HE) staining was used to observe changes of pressure-side periodontal tissue, and changes of RIP3 and MLKL were tested by immunohistochemical staining. Results Compared with group A, expression levels of RIP3 and MLKL in periodontal tissue in group B and C increased firstly and then fell (P<0.05), and the expression of RIP3 increased to the peak on the 5th day, MLKL on the 3rd day. Compared with group B, the expression of RIP3 and MLKL in periodontal tissue in group C were decreased as a whole (P<0.05), and the expression trend was consistent with group B. Conclusion Piezo1 channel may play a role in the mechanotransduction process of orthodontic tooth movement. Blocking the function of Piezo1 channel may reduce the expression of RIP3 and MLKL in pressure-side periodontal tissue, reduce the occurrence of necroptosis and the speed of orthodontic tooth movement.

Key words: orthodontic tooth movement, necroptosis, Piezo1, pressure side

CLC Number:

R783.5

ZHANG Yanyun, LI Yi, WANG He, ZHANG Man, ZHANG Miaomiao. Effect of Piezo1 on RIP3 and MLKL in periodontal tissue of the pressure side during tooth movement in rats[J]. Stomatology, 2022, 42(10): 883-888.

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Effect of Piezo1 on RIP3 and MLKL in periodontal tissue of the pressure side during tooth movement in rats

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