GsMTx4对大鼠牙齿移动过程中痛觉的抑制作用

doi:10.13591/j.cnki.kqyx.2024.11.003

Abstract

Abstract:

Objective To investigate the inhibitory effect of GsMTx4 on pain sensation in rats during tooth movement, open field experiments and immunohistochemical staining by conducting. Methods Fifty-eight male SD rats aged 5-6 weeks were selected and a right maxillary tooth movement model was constructed. The rats were randomly divided into two groups: group A(control group) and group B(GsMTx4 group). They were also divided into 5 subgroups according to 4 h, 1 d, 3 d, 5 d, and 7 d. The left maxillary of the rats in group A was used as group C(blank control group). The open-field test was used to evaluate the pain level of rats. Immunohistochemical staining was used to observe the expression of nerve growth factor(NGF) and calcitonin gene-related peptide(CGRP) in periodontal tissues. Results At 4 h, 1 d, and 3 d, the total distance traveled by rats in group B within 5 minutes in the open-field was higher than that of group A (P<0.05). At 4 h and 1 d, the percentage of time in the center of rats in group B was higher than that in group A(P<0.05). The expressions of NGF and CGRP in groups A and B were higher than those in group C on 1 d, 3 d, 5 d, and 7 d(P<0.05). The expression of NGF and CGRP in group B were lower than those in group A at 4 h, 1 d, and 3 d(P<0.05). Conclusion GsMTx4 can inhibit the expression of NGF and CGRP during tooth movement and relieve the pain caused by tooth movement.

Key words: GsMTx4, orthodontic, pain, NGF, CGRP

CLC Number:

R783.5

WANG Ruofei, SHAO Lixin, LIU Xiaotong, ZHANG Miaomiao. The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats[J]. Stomatology, 2024, 44(11): 815-819.

Figures/Tables 5

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References 23

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组别	4 h	1 d	3 d	5 d	7 d
A组	0.110 9±0.010 7^*	0.132 0±0.017 6^*	0.154 0±0.027 3^*	0.129 9±0.024 0^*	0.126 1±0.020 5^*
B组	0.087 2±0.021 2^*#	0.109 3±0.100 9^*#	0.124 2±0.015 0^*#	0.129 7±0.016 9^*	0.117 9±0.015 8^*
C组	0.048 3±0.188 3	0.048 3±0.188 3	0.048 3±0.188 3	0.048 3±0.188 3	0.048 3±0.188 3

组别	4 h	1 d	3 d	5 d	7 d
A组	0.115 8±0.157 2^*	0.164 6±0.017 9^*	0.187 3±0.028 3^*	0.130 4±0.017 4^*	0.130 0±0.016 3^*
B组	0.084 9±0.015 9^#	0.118 0±0.011 9^*#	0.149 8±0.022 5^*#	0.115 1±0.010 6^*#	0.117 9±0.011 8^*
C组	0.094 8±0.014 4	0.094 8±0.014 4	0.094 8±0.014 4	0.094 8±0.014 4	0.094 8±0.014 4

The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats

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