Abstract: Objective To investigate expressions of genes in osteoblast-like cells derived from bone marrow stem cells (BMSCs) transfected with chitosan nanocarrier containing plasmid-BMP-2 sequences (CNPBs). Methods According to different concentrations of plasmid–BMP-2 (pBMP-2), CNPBs experimental groups were divided into CPB50 concentration group (50 μg/mL pBMP-2), CPB100 concentration group (100 μg/mL pBMP-2) and CPB200 concentration group (200 μg/mL pBMP-2) respectively. Expression levels of specific molecules (Alkaline Phosphatase, ALP; Osteoprotegerin, OPG; Osteocalcin, OC; Osteopontin, OPN) in osteoblast-like cells were detected and analyzed. Results There were higher expression levels of ALP, OPG, OC and OPN genes in osteoblast-like cells in CNPBs groups than control groups, especially in CBP200 group. Conclusion Expression levels of genes in osteoblast-like cells derived from BMSCs transfected with CNPBs can be promoted effectively.