›› 2013, Vol. 33 ›› Issue (3): 151-154.
• Basic and Clinical Research • Previous Articles Next Articles
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Abstract: Abstract: Objective To evaluate the apoptosis of osteocytes to determine whether the C/CSiC composites can be used as implant materials. Methods 8 Japanese bigeared rabbits were selected and randomly divided into group A and group B(n=4).Implant respecitvely 8 pieces of experimental material(C/CSiC composites) and control material(Ti) into femur of the rabbits.Three months later,decalcify the femur specimens with experimental material and control material and immunohistochemical stain them to observe the combination of interface growth and apoptosis of bone tissue.Use Imagepro Plus 6.0 image analysis software to analyse the gray values of brownstained cells,and use SPSS 17.0 statistical software to make ttest analysis of independent samples. Results The experimental animals were anesthetized well and the effect of surgery was good.Implant materials and bone combined tightly.No loosening happened.New bone grew on most of the surfaces of implant materials.Under the light microscopy,cytoplasm of osteocytes was product of immunohistochemistrical positive reaction of caspase3,Bcl2 and Bax,and brown staining of some cell membrane was observed.There was no significant difference in mean grey value between the pure titanium control group and C/CSiC composite experimental group. Conclusions C/CSiC composites and titanium material were implanted in rabbit femurs.3 months later,the apoptosis of the osteoblasts around materials had no significant difference.
CLC Number:
R783.1
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