Abstract: Objective　To explore the effect whether biomembrane was applied or not in maxillary sinus augmentation together with mucosa perforation. Methods　6 sites for implants were chosen in 3 health adult mongrel dogs and were randomly divided into 2 groups. In the control group, models with mucosa perforation at the maxillary sinus floor were established, and the diameter of the mucosa perforation was 5mm. Implants were implanted synchronously. Autogenous bone and artificial bone particles (hydroxyapatite) were also implanted. In the experimental group, models were extablished in the same way. Biological collagen membrane was used to cover the area between sinus floor perforation site and implants. CT photos were taken 1 month and 3 months after the operation. 3 months later, the animals were sacrificed. Observe the osseointegration of implants, mucosa healing at sinus floor, and changes in lengths of implants that protruded into maxillary sinus cavity. Results　1 implant fell off in control group, and a lot of sanies overflowed from maxillary sinus cavity. The rest 4 implants were good in osseointegration. 3 months later, the lengths of the implant exposed increased both in the experimental group and control group, but there was no significant difference between the two groups (P > 0.05). Mucosa at maxillary sinus floor healed well under macroscopic observation. HE staining showed that submucosa at sinus floor in both groups was thickened, and mucosal epithelium ciliated cells disappeared. Conclusions　In this experiment, when mucosa perforation occurred at maxillary sinus floor in maxillary sinus augmentation, biological membrane had no obvious positive effect on the preservation and osteogenesis of artificial bone.