Abstract: Objective To induce the periodontal ligament stem cells in vitro into adipocytes．Methods Periodontal ligament tissue was obtained from extracted orthodontal teeth, then cultivated in nutrient solution. hPDLSC was selected with immunomagnetic beads method. hPDLSC was induced into adipocytes in the adipogenic nutrient solution. Results hPDLSC was selected successfully with immunomagnetic beads method. hPDLSC was induced into adipocytes successfully.Conclusion Immunomagnetic beads method is an effective way to isolate and purify the hPDLSC and hPDLSC was able to transfer into adipocyte.