Abstract: objective The aim of this study was to estimate the amount of fluoride released from three different bonding adhesives by ion selective electrode method.Methods Thirty samples 10mm in diameter and 1 mm in height were divided into 3 groups with 10 in each group. The samples were separately filled into a plastic annular plate,and probes were used to remove overflowing resin at the edge of the mold. After the bonding adhersives were cured, they were stored in 10ml deionized water. The fluoride analysis were carried out using the ion specific electrode for first seven days and there after at every week end for four subsequent weeks. Result There was significant difference among three materials in the amount of released fluoride. Glass inomer cement (TP Orthodontics, USA) released the highest amount of fluoride. There was no significant difference between complex curing glass inomer cement (3M, USA) and Rely-a-bond (Reliance Orthodontic, USA) in the amount of released fluoride. Conclusion All the three bonding adhesives (traditional glass inomer cement, resin reinforced glass inomer cement, and fluoride releasable resin cement ) prove to be effective long term fluoride releasing materials.