Abstract: [Abstract] Objective: To explore the differential protein of oral mucosa between normal controls and OLP patients. Methods: Protein samples were extracted from normal controls and OLP patients and analyzed by scanning Two-dimensional gel electrophoresis maps, searching for possible differential protein, which would be identified by online retrieving collected peptide mass fingerprinting. Results: Of the 28 indentified differential protein spots, 20 protein spots had high expression in OLP and 8 showed low expression in OLP（P＜0.05）. 10 protein spots with high definition were detected with biologic mass spectrometry technique, MALDI-TOF-MS and 5 proteins were identified, of which K2E, vimentin protein, DYHC1 and FGB had a high expression in OLP patients while ApoA1 showed a low expression in OLP. Conclusions: It turns out that there indeed exist differential protein of oral mucosa between normal controls and OLP patients. The identified Specific Protein indicates that K2E may play a role in the pathogenesis of OLP and the occurrence of OLP may be associated with the onset of microcirculatory disturbance and hyperviscosity.

Key words: OLP, Proteome, Two-dimensional gel electrophoresis, MALDI-TOF-MS/MS