›› 2014, Vol. 34 ›› Issue (12): 889-891.

• Basic and Clinical Research • Previous Articles     Next Articles

Effect of Clostridium perfringens enterotoxin on expression of tight junction proteins in rat submandibular glands

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  • Received:2014-07-21 Revised:2014-08-27 Online:2014-12-28 Published:2015-01-02

Abstract: objective To observe the effect of Clostridium perfringens enterotoxin on expression of tight junction proteins in rat submandibular glands Methods recombinant plasmid pET16bHis10C-CPE was constructed, CPE COOH-terminal fragments (C-CPE) were purified. Rat submandibular glands (SMGs) were isolated and then perfused with C-CPE for 3h. After perfusion, the morphology of epithelia cells and expression of ZO-1 and Claudin-4 in SMGs were observed by H.E and immunofluorescence staining. Results After perfusion with C-CPE, no epithelial cells damage was observed and the expression of ZO-1 and Claudin-4 was no change. Conclusions Purified C-CPE fragments have no cytotoxic effect on epithelial cells in SMGs.

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