Abstract: Objective The expression of HNK-1 and Nestin will be checked in the STRO positive DPSC and EMSC which are separated by immunomagnetic bead.To investigate the phenotype characteristics of DPSC and the it s correlation with EMSC to find out the purified cell source for the future study. Methods DPSC and EMSC were obtained with the indirect immunomagnetic bead seperation method.The expression of HNK-1 and Nestin was checked with the indirect immunofluorescent double marker. Results Cell counting was made before and after immunomagnetic bead separation.About 5% DPSCs and 1% EMSCs were STRO-1 positive.HNK-1 was simultaneously expressed in STRO-1 positive DPSC.HNK-1 and Nestin were simultaneously expressed in STRO-1 positive EMSC. Conclusions The STRO-1 positive DPSC expressed the EMSC marker of HNK-1 and Nestin,which shows that both of them are the stem cells from the mesenchymal tissue and the phenotype has the inheritance.

Key words: immunomagnetic bead separation, DPSC, EMSC, STRO-1, HNK-1, Nestin