›› 2014, Vol. 34 ›› Issue (7): 492-496.

• Basic and Clinical Research • Previous Articles     Next Articles

Study on influence of bilateral intermittent nasal obstruction on condylar cartilage cell apoptosis in young rats

  

  • Received:2013-08-05 Revised:2013-09-17 Online:2014-07-28 Published:2014-08-04
  • Contact: Min Zhu E-mail:zminnie@126.com

Abstract: Objective To investigate the condylar cartilage cell apoptosis in young rats with bilateral intermittent nasal obstruction to try exploring the influence of intermittent nasal obstruction on the development of condylar cartilage of children who have to breathe through mouth. Methods Twenty 4-week-old SD rats were employed and divided into two groups. Group A:10 of them had both nostrils occluded by nose plugs on normal oxygen conditions up to 8 hours for 35d and group B served as the control group:10 of them were raised under normal oxygen conditions. The same food and water were offered to the two groups and then the bilateral condylar was extracted to make parafin section for the immumohistochemical staining, including Caspase-3, Bcl-2, Bax protein. Cartilage cell proliferation and apoptosis, the regulatory mechanism of Bcl-2 gene family and its correlation with maxillary growth were observed and analyzed. Results Under the circumstance of bilateral intermittent nasal obstruction, the apoptosis of condylar chondrocytes in Group A increased significantly compared with the control group. The expression of caspase-3, bcl-2, bax protein was significantly higher than the control group. Conclusion Oral breathing caused by nasal obstruction results in the apoptosis in the condyle process cells

Key words: nasal obstruction, condyle process, apoptosis

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