小肠黏膜下层对牙周膜干细胞体外增殖、骨向分化的影响

Abstract

Abstract: Objective To observe the adhesion and growth of human periodontal ligament stem cells (PDLSCs) seeded on small intestinal submucosa (SIS) in vitro and the osteogenic induction of SIS. Methods PDLSCs were successfully isolated and cultured. The compatibility of the biomaterial was evaluated by MTS assay. The osteogenic differentiation of PDLSCs induced by SIS was measured by using Alizarin red staining,the alkaline phosphatase (ALP) activity and reverse transcriptionpolymerase chain reaction (RTPCR) assay. Results The proliferation of PDLSCs in SIS groups was obviously higher than that in the control groups. The higher levels of ALP activity,the larger mineralizing nods were also detected in the experiment groups. RTPCR demonstrated mRNA expression of bone sialoprotein (BSP) and osteocalcin (OCN) in PDLSCs induced by SIS. Conclusions SIS possesses a good cellular compatibility with PDLSCs. It can induce the osteogenic differentiation of PDLSCs.

Key words: periodontal ligament stem cells, small intestinal submucosa, proliferation, osteogenic differentiation

CLC Number:

R780.2

References

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Effects of small intestinal submucosa on proliferation and osteogenic differentiation of human periodontal ligament stem cells in vitr

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