胰岛素样生长因子1经PI3K/Akt信号通道对人牙髓细胞凋亡的抑制调控

Abstract

Abstract: Objective To explore the protein expression of Akt,pAkt,in the human dental pulp cells stimulated by LTA and LPS,and to investigate the protection function of IGF1 to the human dental pulp cell.Methods MTT chromatometry was used to determine cell inhibition rate of HPDCs stimulated by different concentrations LTA and LPS,and to determine cell inhibition rate of HPDCs costimulated by IGF1 and LTA or LPS.The protein expression of Akt,pAkt,in HPDCs were then evaluated by western bloting.Results LTA and LPS inhibited HPDCs viability in a dosedependent fashion.IGF1 had a protection function of HPDCs inhibition caused by LTA and LPS.The protein expression of pAkt(Ser473) in HPDCs costimulated by IGF1 and LTA or LPS was higher than other groups.Conclusions IGF1 had a protection function to HPDCs inhibition caused by LTA and LPS,and this function might have a relationship with PI3K/Aktpathway.

Key words: human dental pulp cell, PI3K/Akt, IGF1, lipopolysaccharide(LPS), lipoteichoic acid(LTA)

CLC Number:

R329.25

References

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Down regulation of IGF1 to dental pulp cell apoptosis through PI3K/Akt pathway

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