›› 2015, Vol. 35 ›› Issue (1): 24-28.

• Basic Research • Previous Articles     Next Articles

Inhibitory effect of EGCG on adipogenic differentiation of BMMSCs via ERK1/2 signaling pathway

  

  • Received:2014-04-14 Revised:2014-06-25 Online:2015-01-28 Published:2015-01-28
  • Contact: lie-hong wu E-mail:wuliehong2011@163.com

Abstract: Objective To determine the effect of epigallocatechin-3-gallate (EGCG) on the proliferation and adipogenic differentiation of bone marrow-derived mesenchymal stromal cells (BMMSCs) and to investigate the underlying molecular mechanism and involved signaling pathway. Methods  To assess the effects of EGCG on the proliferation and adipogenic differentiation of BMMSCs,MTT and Oil red O staining were conducted respectively.The expression levels of key transcriptional factor during adipogenesis,including PPARγ,C/EBPα and C/EBPβ were measured by Real-time fluorescent quantitative RT-PCR.Also,Western blot was performed to confirm the role of ERK1/2 pathway in the inhibitory effects of EGCG on the adipogenic differentiation of BMMSCs. Results  Although low concentrations of EGCG (1-10 mmol/L) exhibited no effects on BMMSCs growth,high levels of EGCG (20-40 mmol/L) significantly decreased the cell proliferative reaction.By inhibiting the expressions of PPARγ,C/EBPα and C/EBPβ,three key transcriptional factors in adipogenesis,EGCG also showed abilities to suppress the adipgenic differentiation of BMMSCs.ERK1/2 pathway was demonstrated to play an essential role in EGCG inhibiting adipogenesis. Conclusions  EGCG can suppress the adipogenic differentiation of BMMSCs via ERK1/2 pathway.

Key words: Epigallocatechin-3-gallate, Bone marrow-derived mesenchymal stromal cells, PPARγ, C/EBPα, C/EBPβ, ERK1/2

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