Abstract: Objective To evaluate the effect of polyhexamethylene guanidine on osteogenic differentiation of rat bone marrow stromal stem cells (BMSCs), and to explore the effect of polyhexamethylene guanidine loaded on TiO2 nanotubes on promoting the osteogenic differentiation of the modified titanium surface.Methods Differentconcentrations of polyhexamethylene guanidine hydrochloride (PHMG-Cl) (1×10-9、1×10-8、1×10-7、1×10-6、1×10-5kg/L) were supplemented into the culture media of rat bone marrow stromal stem cellsseparately.Thecell proliferation was assayed by MTT method. Theosteogenic differentiation markers were analyzed by real-time PCR assay. Then certain concentration of polyhexamethylene guanidine hydrochloride was loaded on the TiO2 nanotubes modified titanium surface, and theosteogenic differentiation markers were analyzed by real-time PCR. ResultsPolyhexamethylene guanidine didn’t inhibit the proliferation of rat BMSCs until the concentration was up to 1×10-5kg/L.Concentration of 1×10-6kg/L enhanced the osteogenic differentiation of rat BMSCs. With polyhexamethylene guanidine hydrochloride being loaded on the TiO2 nanotubes modified titanium surface, the ability of osteogenic differentiation was further enhanced. Conclusion Polyhexamethylene guanidine loaded on TiO2modified titanium surface promotes its osteogenic function in vitro, which indicates that it has the ability to enhance osseointegration in vivo.

Key words: polyhexamethylene guanidine hydrochloride, bone marrow stromal stem cells, osteogenic differentiation