Abstract: Objective To establish a thermal and mechanical quantitative sensory testing (QST) profile at intr- and extra- oral sites and hand and test the site, gender and age differences in healthy Chinese. Methods twenty young subjects and twenty aged subjects participated the study. Cold detection threshold (CDT), warm detection threshold (WDT), cold pain threshold (CPT), heat pain threshold (HPT), mechanical detection threshold (MDT) and mechanical pain threshold (MPT) were measured at four sites: the surface of left hand (Hand) ), the skin of the mental foramen (Chin), the tip of the tongue (Tongue), the mucosa of lower lip (Lip). Statistical analysis was performed using ANOVA with repeated measures to compare the means within and between groups. The bonferroni tests were used to calculate the post-hoc comparisons. Results A significant site effect was observed for CDT, WDT, MDT, and MPT (P＜0.01).There were significant age differences with significantly lower sensitivity for WDT (P＜0.01）, MDT (P＜0.01) , MPT (P＜0.01) and CDT (P=0.01) in the aged group compared to young group. A significantly higher sensitivity for CPT (P＜0.01) and HPT（P=0.03) were detected in women compared to men. Conclusion Application of this thermal and mechanical quantitative sensory testing provide some information for a better understanding of the underlying mechanisms of somatosensory phenotypes and for the better further study of sensory dysfunctions in the intr- and extra oral trigeminal area in healthy human . Key words: quantitative sensory testing(QST); trigeminal system; craniofacial pain

Key words: Quantitative sensory testing(QST), Trigeminal system, Spinal system , Pain