Abstract: Objective To improve the biocompatibility of nanofibrous scaffolds by grafting magnetic nano-particles onto them.Methods Poly (lactic-co-glycolic acid) (PLGA)/ε-caprolactone (PCL)/gelatin (Gel) nanofibrous composite membranes (PPG) were fabricated by electrospinning.And then paramagnetic Fe3O4 nano-particles were assembled to the scaffolds by layer-by-layer (LbL) process.Contact angle meter,electronic universal testing machine,vibrating sample magnetometer were used to characterize the surface topography,hydrophilicity,tensile strength and magnetic properties.Osteoblastic cell line MC3T3-E1 was seeded onto the scaffolds.CCK-8 was used to detect the cell proliferation.Results FESEM showed the structure of the PPG-F scaffold was similar to that of extracellular matrix,and Fe3O4 particles were successfully grafted onto the scaffolds.Vibrating sample magnetometer results indicated that after being coated with Fe3O4 particles,PPG-F membrane was of super paramagnetic property.Both improved hydrophilicity and better cell proliferation were observed in PPG-F group (P<0.05).Conclusions Grafting magnetic Fe3O4 nano-particles onto nanofibrous scaffolds can obviously improve the biocompatibility.

Key words: Electrospinning, Magnetic Fe3O4 nano-particles, LbL self-assembly, Biocompatibility