他米巴罗汀对牙龈卟啉单胞菌抗原刺激RAW264.7细胞的影响

Abstract

Abstract: Objective To investigate the effect of Am80 on murine macrophage RAW264.7 after P.gingivalis antigen stimulation.Methods RAW264.7 cells were stimulated by P.gingivalis antigen and treated with different concentrations of Am80.Cell growth was observed in each group under a phase contrast microscope; the proliferation of antigen-stimulated RAW264.7 cells with different concentrations of Am80 were detected by MTS assay; enzyme-linked immunosorbent assay(ELISA) was used to detect anti-inflammatory factor levels of IL-10 in cell culture supernatant.Results 10 nmol/L Am80 significantly inhibited the proliferation and differentiation of P.gingivalis antigen-stimulated RAW264.7 cells（P<0.001）while Am80 (10 nmol/L) significantly increased the secretion of IL-10 by antigen-stimulated cells (P<0.05); inverted phase contrast microscope showed that Am80 inhibited antigen-stimulated proliferation and differentiation of RAW264.7 cells.Conclusions Am80 (10 nmol/L) can significantly promote P.gingivalis antigen-induced murine macrophages secretion of anti-inflammatory cytokine IL-10,and it may inhibit P.gingivalis antigen-induced inflammation.

Key words: Tamibarotene , macrophage , Interleukin-10, Porphyromonas gingivalis

CLC Number:

R780.2

References

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Effect of Tamibarotene on P.gingivalis antigen-stimulated RAW264.7 cells

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