Nd:YAG激光对鳞状细胞癌CAL-27细胞增殖的影响

Abstract

Abstract: Objective To investigate the biological effect of Nd:YAG laser irradiation on the squamous carcinoma CAL-27 cell proliferation.Methods Squamous carcinoma CAL-27 cells were developed in the vitro,and when they were in the logarithmic phase,the cells were irradiated with different doses of Nd:YAG laser and observed under inverted microscopes.Then,the OD value was measured after 1,3,and 5 days.Results A significant stimulative and inhibitive effect was found respectively of the low and high intensity 1 064 nm laser (P＜0.05) on squamous carcinoma CAL-27 cell proliferation.Conclusions By low intensity 1064 nm Nd:YAG laser,the squamous carcinoma CAL-27 cell proliferation can be stimulated,the cells are morphologically ruled and their density increases.In contrast,the proliferation can be inhibited by high intensity laser,the cells are irregularly shaped and their density decreases.This finding will have a good reference value for the clinical application.

Key words: Nd:YAG laser, squamous carcinoma, proliferation

CLC Number:

R780.2

References

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The effect of Nd: YAG laser irradiation on squamous carcinoma CAL-27 cell proliferation

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