MTA对牙周膜干细胞RANKL/OPG表达水平的影响

Abstract

Abstract: Objective To evaluate the effects of MTA on the expression of receptor activator of NFκB ligand (RANKL) and osteoprotegerin (OPG) in human periodontal ligament stem cells (PDLSCs).Methods First,PDLSCs were cultured separately by enzyme digestion.Then,PDLSCs were cultured in αMEM medium supplemented with different concentrations of MTA conditioned medium and the optimal concentration of MTA conditioned medium was determined by alkaline phosphatase (ALP) activity.PDLSCs cultured in αMEM (control group),MTA conditioned medium containing 2 mg/mL MTA for 3 days and 7 days were respectively collected to evaluate the expression of RANKL and OPG at protein level by Western blot.Results For PDLSCs cultured in MTA conditioned medium for 3 days and 7 days,expression of OPG was all higher than that in control group,while expression of RANKL was all lower than that in control group.Conclusions MTA can regulate the odonto/osteogenic and odonto/osteoclastic potential of PDLSCs through RANKL/OPG system.

Key words: mineral trioxide aggregate, periodontal ligament stem cells, receptor activator of NFκB ligand, osteoprotegerin

CLC Number:

R780.1

References

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Effects of MTA on the expression of RANKL and OPG in human periodontal ligament stem cells

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