Abstract: Objective To observe the effects of heat shock protein 70 (HSP70) induced by glutamine (Gln) on hyperthermia injury to human periodontal ligament cells.Methods The human periodontal ligament cells cultured in vitro were randomly divided into the following four groups:Control Group (Group C:normal human periodontal ligament cells),Gln Pretreatment Group (Group Gln:normal human periodontal ligament cells were added by Gln 15 mmol/L and cultured for 10 h),Severe Hyperthermia Group (Group SH:normal human periodontal ligament cells were cultured at 46 ℃ for 1 h),and Gln Pretreatment +Severe Hyperthermia Group (Group Gln+SH:after normal human periodontal ligament cells were added into Gln 15 mmol/L and cultured for 1 h,they were cultured at 46 ℃ for another hour).The expression of HSP70 was detected by Western Blot.Cell viability was measured using MTT and cytomorphology.Results Western Blot showed that there was higher expression of HSP70 in Group Gln and Group Gln+SH compared to Group C (P<0.05); MTT and cytomorphology indicated that cell viability was higher in Group Gln+SH than in Group SH (P<0.05).Conclusions Gln can induce higher expression of HSP70 in human periodontal ligament cells,thereby reducing hyperthermia injury.

Key words: glutamine, heat shock protein 70, human periodontal ligament cells