Abstract: Objective To explore the expression levels of cyto/chemokines in the gingival crevicular fluid (GCF) of type Ⅱ diabetic mellitus subjects with chronic periodontitis compared with those with simple periodontitis.Methods Fifty-two type Ⅱ diabetic mellitus subjects and forty non-diabetic mellitus subjects with chronic periodontitis were enrolled in this study.The levels of 14 cyto/chemokines were measured in the GCF of healthy and diseased sites of the diabetic and non-diabetic mellitus subjects with chronic periodontitis using Luminex FLEXMAP3D plate reader and Human Cytokine/Chemokine kits.Results The concentrations of eotaxin,macrophage inflammatory protein-1α,granulocyte-macrophage colony-stimulating factor,interleukin (IL)-6,tumour necrosis factor-α and IL-12 were higher at healthy and diseased sites of diabetic mellitus than non-diabetic mellitus subjects.Conclusions Type Ⅱ diabetes mellitus modulated the levels of several cyto/chemokines at both healthy and diseased periodontal sites.It may be a facilitating factor to periodontal disease.

Key words: cytokines/chemokines, chronic periodontitis, type II diabetes mellitus