组织工程化骨修复兔下颌骨缺损同期种植体植入的实验研究

Abstract

Abstract: Objective To investigate osteogenesis and integration of tissue engineered bones repairing rabbit mandibular defect with HA/SLA-Ti and SLA-Ti implants synchronously embedded in them. Methods The tissue engineered bone constructed with rabbit BMSCs, nHAC and PRP was used to repair the full-thickness defect with a size of 15mm×15mm in buccal wall of rabbit mandibular, and HA/SLA-Ti (group A) and SLA-Ti (group B) implants were embedded in them synchronously. After 1, 3, 6 months of operation, specimens were harvested and assessed by means of gross observation, scanning electron microscope observation, histological observation, push-out test and uniaxial pull-out test of implants. Results Visible new bone tissue was formed in bone defect region and fine synostotic interface was formed around implants of group A, but in group B, there were some fibrous tissues between implants and newly formed bone. The results of scanning electron microscope observation showed that new bone was connected with HA/SLA-Ti directly and more tightly in group A than in group B. The results of histological observation showed that bone formation on the surface of HA/SLA-Ti was earlier than that on the surface of SLA-Ti. The results of push-out test and uniaxial pull-out test of implants showed that there was no obvious difference of push in stress force and extract out loading between group A and group B after 1 month of operation. However, after 3 months of operation, compared with group B, push in stress force and extract out loading of implants in group A increased obviously and the difference had statistical significance. Conclusion Dental implants embedded synchronously in tissue engineered bones which were used to repair rabbit mandibular defect could form better synosteosis with newborn bone tissue. The ability of synosteosis of HA/SLA-Ti was better than SLA-Ti, and time of bone healing was shorter than the latter.

Key words: tissue engineered bone, bone defects, immediate implant placement, bone healing, early osseointegration, rabbit

CLC Number:

R782.1

References

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Experimental studies on tissue engineered bones repairing rabbit mandibular defect with synchronous implant embedding

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