感染根管中牙龈卟啉单胞菌fimA基因多态性研究

Abstract

Abstract: Objective: To investigate the distribution of different fimA genotypes of Porphyromonas gingivalis (P. gingivalis) strains in root canals among periapical periodontitis patients. Method: A total of 200 samples of infected root canals with periapical periodontitis were collected. After DNA was extracted from the samples, 16S rRNA gene-based polymerase chain reaction (PCR) method was applied to identify the appearance of P. gingivalis. Among the P. gingivalis-positive samples, fimA type-specific primers were used to amplify the target DNA fragments by PCR, aiming to obtain the information about the distribution of different fimA genotypes. Pearson χ2 test was performed to investigate the correlation between fimA types and symptoms. Result: The presence of P. gingivalis was demonstrated in 48% of total number of infected root canals. A single fimA genotype was detected in 79 samples (82.3%). Combinations of up 2 different genotypes were detected in 12 cases. The distribution of six fimA genotypes among the P. gingivalis-positive samples was as follows: type I, 29.2%; type Ib, 8.3%, type II, 37.5%; type III, 14.6%; type IV, 19.8% and type V, 0.00%. There is no significant correlation between fimA genotypes and symptoms of periapical infection. Conclusion: There is fimA genetic polymorphism of P. gingivalis in infected root canals from different patients with periapical periodontitis. Relevance ratio of type II fimA is the highest, followed by type I and IV.

Key words: Apical periodontitis, infected root canals, Porphyromonas gingivalis, fimA genotype, gene polymorphism

CLC Number:

R780.2

References

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fimA genetic polymorphism of Porphyromonas gingivalis in periapical periodontitis

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